U.S.-Brazil Collaborative Research: Program to Develop a Radiation Hybrid Map of the River Buffalo Genome

这项美国-巴西奖项将支持德克萨斯州农工机械研究基金会的James E.Worack博士与帕拉联邦大学的Paula C.Schneider博士和Estadual Paulista大学的Elisabete J.Aamaral博士合作，通过构建和鉴定辐射杂交(RH)细胞系来绘制河流水牛的特定染色体图谱。他们建议构建一个水牛辐射杂交小组，并开发包含至少60个微卫星和60个保守基因或表达序列标签(EST)的水牛1，2，X和Y染色体框架图。最终将在这些PI的实验室和世界各地的其他实验室中，对整个基因组中的标记进行基因分型。因此，全基因组RH图谱将是该项目开发的资源的产物。这一图谱将是将水牛基因组与其他哺乳动物的基因组进行比较的主要工具，以确定进化重排，并识别和分离与生物学特性有关的基因。这使饲养者能够洞察与农业需求相关的动物改良。

This U.S.-Brazil award will provide support to Dr. James E. Womack, Texas A&M Research Foundation, to collaborate with Dr. Paula C. Schneider, Para Federal University, and Dr. Elisabete J. Aamaral, Universidade Estadual Paulista, on mapping specific chromosomes of river buffalo through the construction and characterization of a panel of radiation hybrid (RH) cell lines. They propose to construct a buffalo radiation hybrid panel and to develop framework maps of buffalo chromosomes 1,2,X, and Y containing at least 60 microsatellites and 60 conserved genes or expressed sequence tags (ESTs). The panel of RH lines generated will eventually be genotyped for markers throughout the genome, both in the laboratories of these Pis and in other labs throughout the world. Thus a whole genome RH map will be a product of the resource developed by this project. This map will be a primary tool for comparison of the buffalo genome to that of other mammals to identify evolutionary rearrangements and for identifying and isolating genes responsible for traits of biological interest. This gives breeders insight into improvement of animals related to agricultural needs.