U.S.-Brazil Collaborative Research: Program to Develop a Radiation Hybrid Map of the River Buffalo Genome

这个美国-巴西奖将提供支持博士詹姆斯E。沃马克，得克萨斯A M研究基金会，与博士保拉C。Schneider，帕拉联邦大学和Elisabete J. Aamaral博士，保利斯塔州立大学，关于通过构建和表征一组辐射杂交（RH）细胞系来绘制河布法罗的特定染色体。 他们建议构建一个布法罗辐射杂交面板，并开发布法罗染色体1，2，X和Y的框架图，其中包含至少60个微卫星和60个保守基因或表达序列标签（EST）。 最终将在这些PI的实验室和世界各地的其他实验室中对产生的RH系组进行基因分型，以获得整个基因组的标记。 因此，全基因组RH图谱将是该项目开发的资源的产物。 该图谱将成为比较布法罗基因组与其他哺乳动物基因组的主要工具，以确定进化重排，并确定和分离负责生物学特性的基因。 这使育种者能够深入了解与农业需求相关的动物改良。

This U.S.-Brazil award will provide support to Dr. James E. Womack, Texas A&M Research Foundation, to collaborate with Dr. Paula C. Schneider, Para Federal University, and Dr. Elisabete J. Aamaral, Universidade Estadual Paulista, on mapping specific chromosomes of river buffalo through the construction and characterization of a panel of radiation hybrid (RH) cell lines. They propose to construct a buffalo radiation hybrid panel and to develop framework maps of buffalo chromosomes 1,2,X, and Y containing at least 60 microsatellites and 60 conserved genes or expressed sequence tags (ESTs). The panel of RH lines generated will eventually be genotyped for markers throughout the genome, both in the laboratories of these Pis and in other labs throughout the world. Thus a whole genome RH map will be a product of the resource developed by this project. This map will be a primary tool for comparison of the buffalo genome to that of other mammals to identify evolutionary rearrangements and for identifying and isolating genes responsible for traits of biological interest. This gives breeders insight into improvement of animals related to agricultural needs.