US-Egypt Cooperative Research: Thermozyme Biotechnology- Study of Production of Lipase/Esterase From Bacillus Stearothermophilus

描述：该奖项是为了表彰佐治亚州亚特兰大佐治亚州立大学生物系的Chung-Dar Lu博士和埃及亚历山大市穆巴拉克科学研究城的Ahmed I El-Diwany博士的合作项目。这项联合研究的目标是研究热酶技术在工业上的应用，重点是以下具体目标：构建嗜热脂肪芽孢杆菌的基因组文库；产生用于密集生产耐热脂肪酶/酯酶的重组菌株；中试生产脂肪酶/酯酶；以及改善脂肪酶/酯酶的性质。范围：这项研究项目将涉及具有互补经验并能够使用合作所需设施的科学家。构建嗜热脂肪芽孢杆菌基因组文库和产生重组菌株的前两个目标将由卢博士和他实验室的一名研究生在佐治亚州立大学解决，那里有必要的设施，而且之前也曾在那里做过类似的工作。脂肪酶/酯酶生产的第三个目标将由El-Diwany博士完成，而改善脂肪酶/酯酶特性的第四个目标将需要两个实验室以及埃及的一家合作工业企业Pharco进行互动。该项目的成功工作可以改进各种酶的生产，这些酶能够经受住高温，而不会对其有用的特性产生不利影响。这种酶很重要，在工业上得到了广泛的应用。这项提议符合INT的目标，即支持美国与外国在互利领域的合作。

Description: This award is for support of a cooperative project by Dr. Chung-Dar Lu of the Department of Biology, Georgia State University in Atlanta, GA, and Dr. Ahmed I El-Diwany, Mubarak City for Scientific Research in Alexandria, Egypt. The goal of this joint research is to study the use of thermozyme technology for industrial applications, with focus on the following specific objectives: construction of genomic libraries of a thermophile, Bacillus stearothermophilus; generation of recombinant strains for intensive production of thermostable lipase/esterase; pilot plant lipase/esterase production; and improvement of lipase/esterase characteristics. Scope: This research project will involve scientists who have complementary experiences and have access to facilities that are needed for the collaboration. The first two objectives of constructing a genome library on B. stearothermophilus and generation of recombinant strains will be addressed at Georgia State by Dr. Lu and a graduate student in his laboratory, where the necessary facilities exist, and where similar work has been done earlier. The third objective of the lipase/esterase production will be carried out by Dr. El-Diwany, while the fourth objective of improvement of lipase/esterase characteristics will require interaction by the two laboratories as well as by PHARCO, a collaborating industrial concern in Egypt. Successful work on this project can lead to improvement in the production of various enzymes that are capable of withstanding high temperatures without adverse effects on their useful properties. Such enzymes are important and are widely used in industry. This proposal meets the INT objective of supporting U.S.-foreign collaboration in areas of mutual benefit.