US-Egypt Cooperative Research: Thermozyme Biotechnology- Study of Production of Lipase/Esterase From Bacillus Stearothermophilus

产品描述：该奖项是为了支持佐治亚州亚特兰大市格鲁吉亚州立大学生物系的Chung-Dar Lu博士和埃及亚历山大市穆巴拉克市科学研究中心的Ahmed I El-Diwany博士的合作项目。 这项联合研究的目标是研究热酶技术在工业应用中的应用，重点是以下具体目标：构建嗜热芽孢杆菌的基因组文库;产生重组菌株，用于密集生产耐热脂肪酶/酯酶;中试工厂脂肪酶/酯酶生产;以及改善脂肪酶/酯酶特性。 适用范围：该研究项目将涉及具有互补经验的科学家，他们可以使用合作所需的设施。 在B上构建基因组文库的前两个目标。在格鲁吉亚，Lu博士和一名研究生将在他的实验室研究嗜热脂肪菌和重组菌株的产生，那里有必要的设施，而且以前也做过类似的工作。 脂肪酶/酯酶生产的第三个目标将由El-Diwany博士执行，而改善脂肪酶/酯酶特性的第四个目标将需要两个实验室以及埃及合作工业公司PHARCO的互动。 该项目的成功工作可以改善各种酶的生产，这些酶能够承受高温，而不会对其有用特性产生不利影响。 这些酶是重要的，并广泛用于工业中。 该提案符合支持美国的INT目标-在互惠互利的领域开展对外合作。

Description: This award is for support of a cooperative project by Dr. Chung-Dar Lu of the Department of Biology, Georgia State University in Atlanta, GA, and Dr. Ahmed I El-Diwany, Mubarak City for Scientific Research in Alexandria, Egypt. The goal of this joint research is to study the use of thermozyme technology for industrial applications, with focus on the following specific objectives: construction of genomic libraries of a thermophile, Bacillus stearothermophilus; generation of recombinant strains for intensive production of thermostable lipase/esterase; pilot plant lipase/esterase production; and improvement of lipase/esterase characteristics. Scope: This research project will involve scientists who have complementary experiences and have access to facilities that are needed for the collaboration. The first two objectives of constructing a genome library on B. stearothermophilus and generation of recombinant strains will be addressed at Georgia State by Dr. Lu and a graduate student in his laboratory, where the necessary facilities exist, and where similar work has been done earlier. The third objective of the lipase/esterase production will be carried out by Dr. El-Diwany, while the fourth objective of improvement of lipase/esterase characteristics will require interaction by the two laboratories as well as by PHARCO, a collaborating industrial concern in Egypt. Successful work on this project can lead to improvement in the production of various enzymes that are capable of withstanding high temperatures without adverse effects on their useful properties. Such enzymes are important and are widely used in industry. This proposal meets the INT objective of supporting U.S.-foreign collaboration in areas of mutual benefit.