US-Egypt Cooperative Research: Thermozyme Biotechnology- Study of Production of Lipase/Esterase From Bacillus Stearothermophilus

该奖项是为了支持乔治亚州亚特兰大佐治亚州立大学生物系的Lu Chung-Dar博士和埃及亚历山大穆巴拉克市的Ahmed I El-Diwany博士的科学研究合作项目。本联合研究的目标是研究热酶技术在工业应用中的应用，重点关注以下具体目标：构建嗜热嗜脂芽孢杆菌的基因组文库；热稳定型脂肪酶/酯酶重组菌株的培养脂肪酶/酯酶生产中试装置；改善脂肪酶/酯酶特性。范围：该研究项目将涉及具有互补经验并能够获得合作所需设施的科学家。前两个目标是建立嗜脂嗜热杆菌基因组文库和重组菌株的产生，Lu博士和他的研究生将在乔治亚州立大学的实验室完成，那里有必要的设施，并且早些时候已经完成了类似的工作。脂肪酶/酯酶生产的第三个目标将由El-Diwany博士进行，而改善脂肪酶/酯酶特性的第四个目标将需要两个实验室以及埃及的合作工业公司PHARCO的相互作用。在这个项目上的成功工作可以改善各种酶的生产，这些酶能够承受高温而不会对其有用特性产生不利影响。这类酶很重要，在工业上得到了广泛的应用。这一提议符合INT在互利领域支持美国与外国合作的目标。

Description: This award is for support of a cooperative project by Dr. Chung-Dar Lu of the Department of Biology, Georgia State University in Atlanta, GA, and Dr. Ahmed I El-Diwany, Mubarak City for Scientific Research in Alexandria, Egypt. The goal of this joint research is to study the use of thermozyme technology for industrial applications, with focus on the following specific objectives: construction of genomic libraries of a thermophile, Bacillus stearothermophilus; generation of recombinant strains for intensive production of thermostable lipase/esterase; pilot plant lipase/esterase production; and improvement of lipase/esterase characteristics. Scope: This research project will involve scientists who have complementary experiences and have access to facilities that are needed for the collaboration. The first two objectives of constructing a genome library on B. stearothermophilus and generation of recombinant strains will be addressed at Georgia State by Dr. Lu and a graduate student in his laboratory, where the necessary facilities exist, and where similar work has been done earlier. The third objective of the lipase/esterase production will be carried out by Dr. El-Diwany, while the fourth objective of improvement of lipase/esterase characteristics will require interaction by the two laboratories as well as by PHARCO, a collaborating industrial concern in Egypt. Successful work on this project can lead to improvement in the production of various enzymes that are capable of withstanding high temperatures without adverse effects on their useful properties. Such enzymes are important and are widely used in industry. This proposal meets the INT objective of supporting U.S.-foreign collaboration in areas of mutual benefit.