Polyamines Metabolism in Pseudomonas Aeruginosa
铜绿假单胞菌中的多胺代谢
基本信息
- 批准号:0316005
- 负责人:
- 金额:$ 13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-08-01 至 2005-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Polyamines (putrescine, spermidine, and spermine) are the major organic polycations found in all living organisms with an essential but poorly understood biological role in cell growth. The importance of polyamines has been implicated in the biosynthesis of macromolecules including RNA, DNA, and protein. Polyamine metabolism is being extensively studied as a target for drug design in treatment of cancers and parasite infection in humans. Development of new antibiotics along this line has lagged behind, mostly due to the lack of information on polyamine catabolism in bacteria. It is the goal of this project to elucidate polyamine catabolism and regulation in P. aeruginosa, which can grow very efficiently on polyamine as the sole source of carbon and nitrogen. A complete genomic sequence annotation and the availability of DNA microarrays of this organism make P. aeruginosa an excellent model organism for the exploration of polyamine catabolism. Preliminary studies with DNA microarrays and comparative genomics approaches have identified more than eighty genes that are differentially induced by exogenous agmatine and/or putrescine. The putative biochemical functions of these genes revealed possible routes of polyamine catabolism and regulation as well as the linkage of polyamine metabolism to other metabolic activities, including organic sulfur uptake/utilization and induction of heat shock response. The specific aims of this project are: (i) to continue the characterization of these genes related to the uptake, catabolism and regulation of putrescine; and (ii) to identify additional genes involved in the catabolism of spermidine and spermine and its regulation. These will be accomplished through the use of transcriptional profiling analyses, computer-aided data mining, genetics manipulation, and enzyme purification and characterization.Findings from this research are expected to provide novel and significant information on polyamines metabolism which could be beneficial to several fields of biology, including pharmaceutical science, bacterial pathogenesis, and agriculture. Students participating in this project will receive cross-disciplinary training in genetics, biochemistry, and bioinformatics, and thus developing an integrative view of bacterial physiology. Further, the concepts of DNA microarrays and data mining for transcriptional profiling analyses will be classroom assignments to help prepare the new generation of students in scientific work and research in the post-genomics era.
多胺(腐胺,亚精胺和精胺)是所有生物体中发现的主要有机聚阳离子,在细胞生长中具有重要但知之甚少的生物学作用。多胺在RNA、DNA和蛋白质等大分子的生物合成中起着重要作用。多胺代谢作为治疗人类癌症和寄生虫感染的药物设计的靶点正在被广泛研究。沿着这条路线开发新的抗生素已经落后,主要是由于缺乏关于细菌中多胺催化剂的信息。铜绿假单胞菌以多胺为唯一碳源和氮源,能高效生长,本研究的目的是阐明多胺对铜绿假单胞菌的催化作用和调控。铜绿假单胞菌基因组序列的完整注释和DNA微阵列的可用性使得铜绿假单胞菌成为探索多胺催化剂的极好模式生物。DNA微阵列和比较基因组学方法的初步研究已经确定了80多个基因的差异诱导外源胍丁胺和/或腐胺。这些基因的生化功能揭示了多胺代谢的可能途径,以及多胺代谢与其他代谢活动的联系,包括有机硫的吸收/利用和热休克反应的诱导。该项目的具体目标是:(一)继续对这些与腐胺的吸收、催化和调节有关的基因进行表征;(二)确定参与亚精胺和精胺催化及其调节的其他基因。这些将通过使用转录谱分析,计算机辅助数据挖掘,遗传操作,酶的纯化和characterization.Findings从这项研究预计将提供新的和重要的信息,多胺代谢,这可能是有益的几个领域的生物学,包括制药科学,细菌的发病机理,和农业。参加该项目的学生将接受遗传学,生物化学和生物信息学的跨学科培训,从而发展细菌生理学的综合观点。此外,DNA微阵列和转录分析数据挖掘的概念将是课堂作业,以帮助新一代学生在后基因组时代的科学工作和研究做好准备。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Chung-Dar Lu其他文献
Functional analysis of the divergent spuABCDEFGH- spuJ operons for polyamine uptake and utilization in Pseudomonas aeruginoa
铜绿假单胞菌中多胺摄取和利用的不同 spuABCDEFGH-spuJ 操纵子的功能分析
- DOI:
- 发表时间:
2002 - 期刊:
- 影响因子:0
- 作者:
Chung-Dar Lu;Yoshifumi Itoh;Yuji Nakada;Ying Jiang - 通讯作者:
Ying Jiang
Unconventional Integration of the bla Gene from Plasmid pIT2 During ISlacZ/hah Transposon Mutagenesis in Pseudomonas aeruginosa PAO1
- DOI:
10.1007/s00284-008-9348-9 - 发表时间:
2009-02-03 - 期刊:
- 影响因子:2.600
- 作者:
Congran Li;Chung-Dar Lu - 通讯作者:
Chung-Dar Lu
Pathways and regulation of bacterial arginine metabolism and perspectives for obtaining arginine overproducing strains
- DOI:
10.1007/s00253-005-0308-z - 发表时间:
2006-04-01 - 期刊:
- 影响因子:4.300
- 作者:
Chung-Dar Lu - 通讯作者:
Chung-Dar Lu
Chung-Dar Lu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Chung-Dar Lu', 18)}}的其他基金
Functional Genomics of D-Amino Acid Metabolism in Pseudomonas Aeruginosa
铜绿假单胞菌 D-氨基酸代谢的功能基因组学
- 批准号:
0950217 - 财政年份:2010
- 资助金额:
$ 13万 - 项目类别:
Continuing Grant
Polyamine and Arginine Metabolism in Pseudomonas aeruginosa
铜绿假单胞菌中的多胺和精氨酸代谢
- 批准号:
0415608 - 财政年份:2004
- 资助金额:
$ 13万 - 项目类别:
Continuing Grant
Arginine Catabolism in Pseudomonas aeruginosa
铜绿假单胞菌中的精氨酸分解代谢
- 批准号:
9985660 - 财政年份:2000
- 资助金额:
$ 13万 - 项目类别:
Continuing Grant
US-Egypt Cooperative Research: Thermozyme Biotechnology- Study of Production of Lipase/Esterase From Bacillus Stearothermophilus
美埃合作研究:Thermozyme生物技术——嗜热脂肪芽孢杆菌生产脂肪酶/酯酶的研究
- 批准号:
9713644 - 财政年份:1997
- 资助金额:
$ 13万 - 项目类别:
Standard Grant
相似国自然基金
一碳代谢(One carbon metabolism)介导上调的 PD1/PDL1 驱动
肿瘤免疫逃逸
- 批准号:2024JJ9491
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
相似海外基金
Influence of carbon source and metabolism on antibiotic resistance and virulence of Pseudomonas aeruginosa during complex in vivo growth modes
复杂体内生长模式下碳源和代谢对铜绿假单胞菌抗生素耐药性和毒力的影响
- 批准号:
529206-2018 - 财政年份:2018
- 资助金额:
$ 13万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Master's
Loving the poison: molecular basis for metabolism of the widely-used food preservative, propionate in Pseudomonas aeruginosa.
热爱毒药:铜绿假单胞菌中广泛使用的食品防腐剂丙酸盐代谢的分子基础。
- 批准号:
BB/R005435/1 - 财政年份:2017
- 资助金额:
$ 13万 - 项目类别:
Research Grant
Pseudomonas detection and metabolism of sphingosine
假单胞菌检测和鞘氨醇代谢
- 批准号:
8581641 - 财政年份:2012
- 资助金额:
$ 13万 - 项目类别:
Pseudomonas detection and metabolism of sphingosine
假单胞菌检测和鞘氨醇代谢
- 批准号:
8760307 - 财政年份:2012
- 资助金额:
$ 13万 - 项目类别:
Pseudomonas detection and metabolism of sphingosine
假单胞菌检测和鞘氨醇代谢
- 批准号:
9170956 - 财政年份:2012
- 资助金额:
$ 13万 - 项目类别:
Pseudomonas detection and metabolism of sphingosine
假单胞菌检测和鞘氨醇代谢
- 批准号:
8417470 - 财政年份:2012
- 资助金额:
$ 13万 - 项目类别:
Functional Genomics of D-Amino Acid Metabolism in Pseudomonas Aeruginosa
铜绿假单胞菌 D-氨基酸代谢的功能基因组学
- 批准号:
0950217 - 财政年份:2010
- 资助金额:
$ 13万 - 项目类别:
Continuing Grant
Adaptation of Pseudomonas aeruginosa in virulence and metabolism: requirements for chronic lung infection in cystic fibrosis patients
铜绿假单胞菌毒力和代谢的适应:囊性纤维化患者慢性肺部感染的要求
- 批准号:
71800471 - 财政年份:2008
- 资助金额:
$ 13万 - 项目类别:
Priority Programmes
Metabolism of Pseudomonas aeruginosa under conditions of persistent infection of the cystic fibrosis lung
囊性纤维化肺持续感染条件下铜绿假单胞菌的代谢
- 批准号:
71800214 - 财政年份:2008
- 资助金额:
$ 13万 - 项目类别:
Priority Programmes
Polyamine and Arginine Metabolism in Pseudomonas aeruginosa
铜绿假单胞菌中的多胺和精氨酸代谢
- 批准号:
0415608 - 财政年份:2004
- 资助金额:
$ 13万 - 项目类别:
Continuing Grant