LEXEN: Protistan Biodiversity in Antarctic Marine Ecosystems: Molecular Biological and Traditional Approaches
LEXEN:南极海洋生态系统中的原生生物多样性:分子生物学和传统方法
基本信息
- 批准号:9714299
- 负责人:
- 金额:$ 35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-09-15 至 2002-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
*** Caron 9714299 The analysis of microbial biodiversity of extreme environments is difficult because traditional methods for examining diversity are often ineffective for assessing species richness within these communities. Additional difficulties arise due to the difficulties of recreating and maintaining pertinent environmental features during sample collection and procession. This study focuses on the protistan assemblages (algae and protozoa) in the sea ice, sediment and ocean environments of the Ross Sea, Antarctica. The identification of protistan species in natural assemblages traditionally has entailed direct microscopical analyses as well as enrichment and culture techniques for assessing biodiversity. Determination of diversity for these assemblages in therefore susceptible to biases as a consequence of sampling, enrichment and culture, as well as selective losses due to sample preservation and concentration for microscopy. The goals of this project are: (1) to develop and apply molecular biological approaches to assess species diversity of small protists (algae and protozoa smaller than 100 micrometers) in ocean water, sea ice and sediment environments and (2) to obtain baseline physiological information on the growth rates, feeding rates and growth efficiencies of cultured protozoa under pertinent temperature regimes. Molecular biological studies will involve the use of PCR-based protocols to examine small subunit ribosomal RNA gene (srDNA) diversity. Approaches and techniques developed will be applicable to any other water body or sediment and would provide a means to examine the representativeness of protistan cultures in extant culture collections. ***
* 卡隆酒店9714299 极端环境中微生物生物多样性的分析是困难的,因为传统的多样性检查方法往往是无效的评估这些社区内的物种丰富度。由于在样品收集和处理期间难以重建和维持相关的环境特征,因此出现了其他困难。本研究主要研究南极罗斯海海冰、沉积物和海洋环境中的原生生物组合(藻类和原生动物)。 在自然群落中识别原生生物物种传统上需要直接的显微镜分析以及评估生物多样性的富集和培养技术。因此,这些组合的多样性的测定容易受到偏差的采样,富集和文化,以及选择性的损失,由于样品保存和浓缩显微镜。该项目的目标是:(1)开发和应用分子生物学方法,评估海水、海冰和沉积物环境中小型原生生物(小于100微米的藻类和原生动物)的物种多样性;(2)获取有关温度条件下培养原生动物的生长率、摄食率和生长效率的基线生理信息。分子生物学研究将涉及使用PCR为基础的协议,检查小亚基核糖体RNA基因(srDNA)的多样性。开发的方法和技术将适用于任何其他水体或沉积物,并将提供一种手段,以检查现存的文化收藏的原生生物文化的代表性。 ***
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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David Caron其他文献
(190) A Curious Case of Psychosis Post-Liver Transplant
- DOI:
10.1016/j.jaclp.2022.10.192 - 发表时间:
2022-11-01 - 期刊:
- 影响因子:
- 作者:
David Caron;Mélanie Bilbul;Laury Chamelian - 通讯作者:
Laury Chamelian
David Caron的其他文献
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{{ truncateString('David Caron', 18)}}的其他基金
Experimental studies to understand and evaluate acclimation of marine plankton assemblages to increased CO2 and temperature.
旨在了解和评估海洋浮游生物群对增加的二氧化碳和温度的适应的实验研究。
- 批准号:
0962309 - 财政年份:2010
- 资助金额:
$ 35万 - 项目类别:
Standard Grant
MO: Assembly of Marine Microbial Communities
MO:海洋微生物群落的组装
- 批准号:
0703159 - 财政年份:2007
- 资助金额:
$ 35万 - 项目类别:
Continuing Grant
Collaborative Research: Do Crustacean Zooplankton Play a Pivotal Role in Structuring Heterotrophic Plankton Communities?
合作研究:甲壳类浮游动物在构建异养浮游生物群落中发挥关键作用吗?
- 批准号:
0542456 - 财政年份:2006
- 资助金额:
$ 35万 - 项目类别:
Continuing Grant
Collaborative Research: Protistan Abundance, Diversity and Activity in the Deep-Sea and at Hydrothermal Vents
合作研究:深海和热液喷口的原生生物丰度、多样性和活动
- 批准号:
0550829 - 财政年份:2006
- 资助金额:
$ 35万 - 项目类别:
Standard Grant
Collaborative Research: Comparative and Quantitative Studies of Protistan Molecular Ecology and Physiology in Coastal Antarctic Waters
合作研究:南极沿海水域原生生物分子生态学和生理学的比较和定量研究
- 批准号:
0125437 - 财政年份:2002
- 资助金额:
$ 35万 - 项目类别:
Standard Grant
Collaborative Research: Seasonal Contribution of Nano- and Microzooplankton to Antarctic Food Web Structure in the Ross Sea
合作研究:罗斯海纳米和微型浮游动物对南极食物网结构的季节性贡献
- 批准号:
0049008 - 财政年份:2000
- 资助金额:
$ 35万 - 项目类别:
Continuing Grant
Collaborative Research: Seasonal Contribution of Nano- and Microzooplankton to Antarctic Food Web Structure in the Ross Sea
合作研究:罗斯海纳米和微型浮游动物对南极食物网结构的季节性贡献
- 批准号:
9633703 - 财政年份:1996
- 资助金额:
$ 35万 - 项目类别:
Continuing Grant
Fitting Mixotrophy into the Microbial Loop Paradigm: Advantages and Consequences of Mixotrophic Behavior Among Phagotrophic Algae
将混合营养融入微生物循环范式:吞噬藻类混合营养行为的优点和后果
- 批准号:
9508571 - 财政年份:1995
- 资助金额:
$ 35万 - 项目类别:
Continuing Grant
JGOFS: Carbon Flow Through The Microbial Loop in the Arabian Sea: Heterotrophic Nanoplankton Growth Rates and Picoplankton Grazing
JGOFS:阿拉伯海微生物循环中的碳流:异养纳米浮游生物生长率和超微型浮游生物放牧
- 批准号:
9310693 - 财政年份:1994
- 资助金额:
$ 35万 - 项目类别:
Continuing Grant
Collaborative Research: Reproductive Periodicity in Planktonic Sarcodines: Implications for Temporal Variability in Abundance and Flux
合作研究:浮游肉碱的繁殖周期性:对丰度和通量的时间变异性的影响
- 批准号:
9314533 - 财政年份:1994
- 资助金额:
$ 35万 - 项目类别:
Continuing Grant
相似海外基金
Relative Informativeness of Endosymbiont-Derived Nuclear Genes in Reconstructing Protistan Phylogenies
内共生体衍生的核基因在重建原生生物系统发育中的相对信息量
- 批准号:
554089-2020 - 财政年份:2020
- 资助金额:
$ 35万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Master's
Development and demonstration of quantification method for bacterivorous character of in situ protistan community
原位原生生物群落食菌特性定量方法的开发和示范
- 批准号:
17K18000 - 财政年份:2017
- 资助金额:
$ 35万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Protistan, prokaryotic, and viral processes at the San Pedro Ocean Time-series
圣佩德罗海洋时间序列中的原生生物、原核生物和病毒过程
- 批准号:
1737409 - 财政年份:2017
- 资助金额:
$ 35万 - 项目类别:
Standard Grant
Factors driving the distribution and diversity of protistan plant pathogens in grasslands and forests of the Biodiversity Exploratories (PATHOGEN)
生物多样性探索区草原和森林原生植物病原体分布和多样性的驱动因素(PATHOGEN)
- 批准号:
325199846 - 财政年份:2017
- 资助金额:
$ 35万 - 项目类别:
Infrastructure Priority Programmes
Dynamics of Protistan Grazers: Diversity, Abundance and Prey Relations
原生食草动物的动态:多样性、丰度和猎物关系
- 批准号:
1434440 - 财政年份:2014
- 资助金额:
$ 35万 - 项目类别:
Standard Grant
Protistan microflora and microfauna in usual lakes and ponds
常见湖泊和池塘中的原生生物微生物区系和微型动物群
- 批准号:
25440202 - 财政年份:2013
- 资助金额:
$ 35万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Resolving the trophic connection between protistan grazers and mesozooplankton in marine food webs using amino acid-specific stable isotope analyses
使用氨基酸特异性稳定同位素分析解决海洋食物网中原生食草动物和中型浮游动物之间的营养联系
- 批准号:
1260055 - 财政年份:2013
- 资助金额:
$ 35万 - 项目类别:
Standard Grant
Protistan grazing and viral infection of marine picoplankton: a role for the host cell surface?
海洋超微型浮游生物的原生生物放牧和病毒感染:宿主细胞表面的作用?
- 批准号:
NE/J02273X/1 - 财政年份:2012
- 资助金额:
$ 35万 - 项目类别:
Research Grant
Protistan grazing and viral infection of marine picoplankton: a role for the host cell surface?
海洋超微型浮游生物的原生生物放牧和病毒感染:宿主细胞表面的作用?
- 批准号:
NE/J020745/1 - 财政年份:2012
- 资助金额:
$ 35万 - 项目类别:
Research Grant
Dimensions: Pattern and Process in Marine Bacterial, Archaeal, and Protistan Biodiversity, and Effects of Human Impacts
维度:海洋细菌、古菌和原生生物多样性的模式和过程,以及人类影响的影响
- 批准号:
1136818 - 财政年份:2011
- 资助金额:
$ 35万 - 项目类别:
Standard Grant