Revision of the phycoerythrin 545-containing cryptophytes

含藻红蛋白545的隐植物的修订

• 批准号: 118169271
• 负责人: Privatdozentin Dr. Kerstin Hoef-Emden
• 金额: --
• 依托单位: Arbeitsgruppe Hoef-Emden
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/118169271?language=en
• 关键词: Revision; phycoerythrin; 545; containing; cryptophytes

The Cryptophyceae consist predominantly of photosynthetic flagellates that successfully conquered marine as well as freshwater habitats. They are ecologically important as prey for several planktic predators (e.g. clliate, copepod or dinoflagellate species). Although cryptophytes as such can be easily identified due to a unique combination of characters, dimorphisms and a lack of species-specific morphological characters cause problems in identification at lower classification levels. This resulted in an inconsistent systematic seriously compromising biodiversity surveys of cryptophyte taxa. This project aims at establishing a sound systematics of the predominantly marine phycoerythrin 545-containing cryptophyte genera. All examinations are based on clonal strains. To examine relationships among the cryptophyte lineages, phylogenetic trees are inferred from combined data sets of ribosomal DNA sequences of two genomes, nucleus and nucleomorph. By mapping morphological characters onto phylogenetic trees, genus- or species-specific characters can be identified. Since sexual reproduction cannot be induced in cryptophyte cultures, compensating base pair changes in the internal transcribed spacer 2 of the nuclear ribosomal operon will be used as indirect markers to predict putative biological species. The taxonomic revisions include molecular signatures as diagnostic characters to allow for an identification of genetically distinct but morphologically ambiguous taxa.

隐藻纲主要由光合鞭毛虫组成，它们成功地征服了海洋和淡水栖息地。它们是生态上重要的几种浮游掠食者（如clliate，桡足类或甲藻物种）的猎物。虽然隐芽植物本身可以很容易地识别，由于一个独特的组合的字符，二型和缺乏种特异性的形态特征造成的问题，在较低的分类水平的识别。这导致了一个不一致的系统严重损害的生物多样性调查的隐藻类群。本项目旨在建立一个健全的系统学的主要海洋含藻红蛋白545的隐藻属。所有检查均基于克隆菌株。为了研究隐孢子植物谱系之间的关系，系统发育树推断从两个基因组，核和核型的核糖体DNA序列的组合数据集。通过将形态特征映射到系统发育树上，可以识别属或种特异性特征。由于在隐藻培养物中不能诱导有性生殖，因此核核糖体操纵子的内部转录间隔区2中的补偿碱基对变化将被用作间接标记来预测推定的生物物种。分类学修订包括分子标记作为诊断特征，以允许识别遗传上不同但形态上模糊的分类群。