CAREER: A Polymerase Switch in the Synthesis of rRNA in Yeast

职业生涯：酵母 rRNA 合成中的聚合酶开关

• 批准号: 9722253
• 负责人: Heather Conrad-Webb
• 金额: $ 49.73万
• 依托单位: Texas Woman's University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722253&HistoricalAwards=false
• 关键词: CAREER; Polymerase; Switch; Synthesis; rRNA

9722253 Conrad-Webb As we achieve greater understanding of the molecular basis of cellular structure and function, the distinctions among genetics, molecular biology and cellular biology fade. Undergraduate students, who usually take separate courses in these disciplines, often compartmentalize information to the specific course and do not recognize the interdependence of these cellular processes. Such fragmentation impedes the recognition of basic biological principles. This project includes three educational objectives: a three semester course which integrates genetics, molecular and cellular biology and emphasizes cellular interactions; a corresponding two semester laboratory course to reinforce fundamental principles by conducting basic experiments; and exposure of students to the process of discovery through independent research. The independent research will focus on the study of a polymerase switch in the synthesis of rRNA. In respiratory deficient strains of Saccharomyces cerevisiae, rRNA is synthesized by both RNA polymerase I and RNA polymerase II. In these cells, a cryptic pol II rDNA promoter that overlaps the pol I promoter is used to synthesize an equivalent 35S precursor rRNA that can alleviate the requirement for pol I. This novel finding introduces new questions in the regulation of rRNA synthesis and is particularly well suited to illustrating the role of scientific inquiry in changing perceptions of cellular function. The objectives of this research are to: 1. Define the sequence signals for pol II transcription of rDNA. 2. Identify regulatory factors required for pol II transcription of rDNA. 3. Investigate the function of pol II rRNA. Dissecting the role of poll II transcription of rRNA presents an intriguing problem for the introduction of students to the role of scientific discovery in expanding our understanding multiple cellular processes. The switch in rRNA synthesis from pol I to pol II involves the interactions of multiple cellular processes including : interorganellar communication, transcriptional activation and silencing, and preferential excision and replication of rDNA episomal copies. Students will be investigating these interactions by synthesizing the various cellular processes that intertwine to produce pol II synthesis of rRNA. %%% As we achieve greater understanding of the molecular basis of cellular structure and function, the distinctions among genetics, molecular biology and cellular biology fade. This project will include the development of a three semester course to integrate the information usually acquired in separate courses in genetics, cell and molecular biology. Emphasis will be placed on the synthesis rather than compartmentalization of information, and the interactions of cellular processes as a framework for problem solving. Furthermore, by participation in laboratory exercises and in the pursuit of independent research, students will witness first hand the process of scientific inquiry. In respiratory deficient strains of yeast is synthesized by both RNA polymerase I and RNA polymerase II. Dissecting the role of pol II transcription of rRNA presents an intriguing problem which will be used to introduce students to the role of scientific discovery in expanding our understanding multiple cellular processes. Students will be investigating these interactions by synthesizing the various cellular processes that intertwine to produce pol II synthesis of rRNA. ***

小行星9722253 随着我们对细胞结构和功能的分子基础有了更深入的了解，遗传学、分子生物学和细胞生物学之间的区别逐渐消失。 本科生，谁通常采取单独的课程在这些学科，往往划分信息的具体课程，并没有认识到这些细胞过程的相互依赖性。 这种分裂阻碍了对基本生物学原理的认识。 该项目包括三个教育目标：三个学期的课程，整合遗传学，分子和细胞生物学，并强调细胞相互作用;相应的两个学期的实验室课程，通过进行基本实验来加强基本原则;学生通过独立研究发现的过程。 这项独立的研究将集中在rRNA合成中聚合酶开关的研究上。 在酿酒酵母的呼吸缺陷型菌株中，rRNA由RNA聚合酶I和RNA聚合酶II合成。 在这些细胞中，与pol I启动子重叠的隐蔽pol II rDNA启动子用于合成可减轻对pol I的需求的等效35 S前体rRNA。 这一新的发现在rRNA合成的调节中引入了新的问题，特别适合于说明科学探究在改变细胞功能认知中的作用。 本研究的目的是：1。定义pol II转录rDNA的序列信号。 2.鉴定pol II转录rDNA所需的调节因子。 3.研究pol II rRNA的功能。 剖析poll II转录rRNA的作用提出了一个有趣的问题，为学生介绍科学发现的作用，扩大我们的理解多个细胞过程。 rRNA合成从pol I到pol II的转换涉及多个细胞过程的相互作用，包括：细胞器间通讯，转录激活和沉默，以及rDNA附加体拷贝的优先切除和复制。 学生们将通过合成各种细胞过程来研究这些相互作用，这些细胞过程相互作用产生rRNA的pol II合成。 随着我们对细胞结构和功能的分子基础有了更深入的了解，遗传学、分子生物学和细胞生物学之间的区别逐渐消失。 该项目将包括一个三个学期的课程，整合通常在遗传学，细胞和分子生物学单独课程获得的信息的发展。 重点将放在信息的综合而不是划分，以及细胞过程的相互作用作为解决问题的框架。 此外，通过参与实验室练习和追求独立研究，学生将亲眼目睹科学探究的过程。 在酵母的呼吸缺陷型菌株中，RNA聚合酶I和RNA聚合酶II都是合成的。 剖析rRNA的pol II转录的作用提出了一个有趣的问题，将用于向学生介绍科学发现在扩大我们对多个细胞过程的理解中的作用。 学生们将通过合成各种细胞过程来研究这些相互作用，这些细胞过程相互作用产生rRNA的pol II合成。 ***