CAREER: The Research Integrated Curriculum Involving A Molecular Study of Retrotransposition

职业：涉及逆转座分子研究的研究综合课程

• 批准号: 9722274
• 负责人: Jill Keeney
• 金额: $ 43.27万
• 依托单位: Juniata College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722274&HistoricalAwards=false
• 关键词: CAREER; Research; Integrated; Curriculum; Involving

Keeney 9722274 The educational objectives of this CAREER award are to expose students to the excitement of scientific research from the high school through the college level. This is being achieved by: 1. Preparing undergraduate students for engaging fully in research through development of an investigative laboratory course, Molecular Techniques, based on the research in the principal investigator's laboratory. 2. Providing the capstone experience of senior research and providing the opportunity for students to present their research results. 3. Involving local high school teachers in the research project and assisting them in more effectively relating the research process to their students through development and implementation of appropriate curricular exercises. Developed exercises will be distributed through the successful Science in Motion Outreach Project in place at Juniata College. 4. Continuing building a strong Biochemistry/Cell and Molecular Biology Program through advising and integration of educational activities which expose students to the process and complexity of research. The research involves using a genetic system to study the mechanism of reverse transcription in yeast retroelements. The reverse transcriptase (RT) enzyme of these elements, like the RT enzymes of retroviruses, employ a host-encoded tRNA molecule as a primer in the first step of copying their RNA into DNA. The resulting DNA can then be incorporated into the host genome, allowing for further replication. The research is appropriate for undergraduates due to the relative simplicity of techniques required to conduct yeast genetic studies. The research objectives are to: 1. Use saturated mutagenesis of the tRNA gene, initiated in the Molecular Techniques laboratory, to identify residues important for recognition by RT enzyme. 2. Use identified tRNA mutations to screen a panel of RT mutations in vivo for the rescue of transposition, thus identifying the amino acid residues in the RT enzyme required f or recognition of the tRNA primer. 3. Purify mutant RT enzyme for collaborative biochemical studies of interaction with primer tRNA. Implementation of the research objectives relates directly to the institutional goal of building a strong program which includes an opportunity for research experience. The educational objectives will strengthen this goal by integrating into the curriculum, beginning in high school, the opportunities for students to experience the excitement of discovery. Students choosing to pursue a capstone research project as college seniors will be well prepared, assuring a positive and productive experience with cutting-edge research.

Keeney 9722274这个职业奖项的教育目标是让学生从高中到大学都能感受到科学研究的兴奋。这是通过以下方式实现的：1.在首席调查员实验室的研究基础上，通过开发一门名为分子技术的调查实验室课程，为本科生全面参与研究做好准备。2.提供高级研究的顶峰体验，为学生提供展示研究成果的机会。3.让当地高中教师参与研究项目，并通过制定和实施适当的课程练习，帮助他们更有效地将研究过程与学生联系起来。开发的练习将通过朱尼亚塔学院成功的运动中科学推广项目分发。4.继续建立一个强大的生物化学/细胞和分子生物学课程，通过建议和整合教育活动，使学生接触到研究的过程和复杂性。这项研究涉及使用遗传系统来研究酵母逆转录元件中的逆转录机制。这些元件的逆转录酶(RT)酶，就像逆转录病毒的RT酶一样，在将其RNA复制到DNA的第一步中使用宿主编码的tRNA分子作为引物。然后，产生的DNA可以被整合到宿主基因组中，从而允许进一步的复制。这项研究适合本科生，因为进行酵母基因研究所需的技术相对简单。本研究的目的是：1.利用分子技术实验室启动的tRNA基因饱和诱变来鉴定与RT酶识别有关的重要残基。2.利用已鉴定的tRNA突变，在体内筛选一组RT突变，以挽救转座，从而鉴定RT酶中识别tRNA引物所需的氨基酸残基。3.纯化突变型RT酶，用于与tRNA相互作用的协同生化研究。研究目标的实施直接关系到建立一个强有力的方案的机构目标，其中包括获得研究经验的机会。教育目标将通过将学生从高中开始体验发现的兴奋的机会纳入课程来加强这一目标。作为大学大四学生，选择从事顶石研究项目的学生将做好充分准备，确保获得积极和富有成效的前沿研究体验。