U.S.-Czech Research on Molecular and Biochemical diversity of Chlorobenzoate Degrading Bacteria

这项由威斯康星大学麦迪逊分校的William Hickey和布拉格捷克微生物研究所的Vladimir Brenner共同开展的美捷研究项目，将对氯苯酸酯生物降解基因（ohb）进行表征：铜绿假单胞菌菌株JB2和恶臭假单胞菌菌株P111。研究人员期望，对多氯联苯矿化的一个关键方面的研究将有助于更好地理解多氯联苯对土壤细菌代谢途径进化的影响。合作将包括通过基因组文库的构建/筛选、核苷酸序列分析和代谢研究来分离和表征ohb基因。重组多氯联苯矿化菌株将通过广泛宿主范围自杀传递系统构建，将ohb基因引入从捷克共和国多氯联苯污染地点分离的联苯降解物中。然后将使用多氯联苯混合物测试这些菌株在土壤中矿化多氯联苯的功效。总体而言，这些结果有望为开发具有成本效益的多氯联苯生物修复方法提供见解，多氯联苯是一类重要的环境污染物。这个代谢生物化学项目通过使美国和东欧的顶尖专家在共同感兴趣和能力强的领域结合互补的人才和集中研究资源，实现了推进科学知识的计划目标。?？

INT 9722620 Hickey This U.S-Czech research project between William Hickey of the University of Wisconsin-Madison and Vladimir Brenner of the Czech Institute of Microbiology, Prague, will characterize chlorobenzoate biodegradation genes (ohb): Pseudomonas aeruginosa strain JB2 and Pseudomonas putida strain P111. The researchers expect that this examination of a key aspect of PCB mineralization will lead to a better understanding of the impact of PCBs on the evolution of metabolic pathways in soil bacteria. The collaborative effort will involve isolation and characterization of the ohb genes by genomic library construction/screening, nucleotide sequence analysis and metabolism studies. Recombinant PCB-mineralizing strains will be constructed by using a broad host-range suicide delivery system to introduce the ohb genes into biphenyl degraders isolated from PCB contaminated sites in the Czech Republic. These strains will then be tested for efficacy in mineralizing PCBs in soil by using PCB mixtures. Overall, results are expected to contribute insight in developing cost effective approaches for bioremediation of PCBs, an important class of environmental pollutants. This project in metabolic biochemistry fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of strong mutual interest and competence. ??