U.S.-Czech Research on Molecular and Biochemical diversity of Chlorobenzoate Degrading Bacteria

美国-捷克关于氯苯甲酸酯降解细菌分子和生化多样性的研究

• 批准号: 9722620
• 负责人: William Hickey
• 金额: $ 2.96万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722620&HistoricalAwards=false
• 关键词: U.S.; Czech; Research; Molecular; Biochemical

INT 9722620 Hickey This U.S-Czech research project between William Hickey of the University of Wisconsin-Madison and Vladimir Brenner of the Czech Institute of Microbiology, Prague, will characterize chlorobenzoate biodegradation genes (ohb): Pseudomonas aeruginosa strain JB2 and Pseudomonas putida strain P111. The researchers expect that this examination of a key aspect of PCB mineralization will lead to a better understanding of the impact of PCBs on the evolution of metabolic pathways in soil bacteria. The collaborative effort will involve isolation and characterization of the ohb genes by genomic library construction/screening, nucleotide sequence analysis and metabolism studies. Recombinant PCB-mineralizing strains will be constructed by using a broad host-range suicide delivery system to introduce the ohb genes into biphenyl degraders isolated from PCB contaminated sites in the Czech Republic. These strains will then be tested for efficacy in mineralizing PCBs in soil by using PCB mixtures. Overall, results are expected to contribute insight in developing cost effective approaches for bioremediation of PCBs, an important class of environmental pollutants. This project in metabolic biochemistry fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of strong mutual interest and competence. ??

INT 9722620 Hickey 威斯康星大学麦迪逊分校的William Hickey和布拉格捷克微生物学研究所的弗拉基米尔布伦纳之间的这项美国-捷克研究项目将表征氯苯甲酸盐生物降解基因（ohb）：铜绿假单胞菌菌株JB 2和恶臭假单胞菌菌株P111。 研究人员预计，对PCB矿化的一个关键方面的研究将有助于更好地了解PCB对土壤细菌代谢途径演变的影响。 合作的努力将包括分离和表征的ohb基因的基因组文库建设/筛选，核苷酸序列分析和代谢研究。 重组多氯联苯矿化菌株将通过使用广泛的宿主范围的自杀传递系统，引入到联苯降解分离的多氯联苯污染的网站在捷克共和国的ohb基因。 然后将测试这些菌株在使用多氯联苯混合物矿化土壤中的多氯联苯方面的效力。 总体而言，结果预计将有助于深入了解开发成本效益的方法，多氯联苯，一类重要的环境污染物的生物修复。 代谢生物化学的这个项目实现了通过使美国和东欧的领先专家能够联合收割机互补人才和在强烈的共同兴趣和能力领域汇集研究资源来推进科学知识的计划目标。 ??