CAA: Analysis of Vertebrate Telomere Proteins Using Gene Targeting

CAA：使用基因靶向分析脊椎动物端粒蛋白

• 批准号: 9722790
• 负责人: Carolyn Price
• 金额: $ 3万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9722790&HistoricalAwards=false
• 关键词: CAA; Analysis; Vertebrate; Telomere; Proteins

9722790 Price The goal of this project is to develop a vertebrate cell culture system for making targeted disruptions of telomere protein genes. This culture system will allow researchers to explore the role different telomere proteins play in telomere function. Vertebrate telomeres are large nucleoprotein complexes and these complexes are expected to be comprised of many different proteins. Targeted disruptions of the TRF1 gene, the gene encoding the first vertebrate telomere protein identified, will be made and the null phenotype analyzed. A chicken B-cell line that has an unusually high frequency of homologous recombination will be used. Mutant versions of the TRF1 gene will be used to complement the null phenotype in order to dissect and understand the function of the protein. This Career Advancement Award to Dr. Carolyn Price will allow her to gain the technical expertise and the in-depth knowledge necessary for a study of vertebrate telomere function. This sabbatical experience will allow her to switch her research program in her own laboratory from investigations into the function of telomeres in ciliated protozoa to similar studies in vertebrates.

这个项目的目标是开发一种脊椎动物细胞培养系统，用于有针对性地破坏端粒蛋白基因。这种培养系统将使研究人员能够探索不同的端粒蛋白在端粒功能中的作用。脊椎动物的端粒是大的核蛋白复合物，这些复合物预计由许多不同的蛋白质组成。将有针对性地破坏TRF1基因，该基因编码第一个鉴定的脊椎动物端粒蛋白，并分析零表型。将使用具有异常高的同源重组频率的鸡b细胞系。TRF1基因的突变版本将用于补充零表型，以便解剖和了解蛋白质的功能。Carolyn Price博士获得的职业发展成就奖将使她获得脊椎动物端粒功能研究所需的技术专长和深入知识。这段休假经历将使她能够将自己实验室的研究项目从对纤毛原生动物端粒功能的研究转向对脊椎动物的类似研究。