Characterization of Arabidopsis thaliana Mutants with Enhanced Disease Susceptibility

小行星9723493 分离出具有增强的疾病易感性（eds）表型的拟南芥突变体，并且一些被鉴定为NPR 1的等位基因，而另一些被鉴定为PAD基因的等位基因。已知NPR 1基因参与系统获得性抗性的调节，而PAD基因参与植物抗毒素合成（一种已知的防御反应）。此外，EDS4基因控制防御基因PR1和PR5响应细菌病原体感染的表达。 因此，eds突变定义了在植物防御病原体攻击中具有重要作用的基因。eds突变体的鉴定将有助于发现在植物抗病性中起主要作用的新基因。EDS基因将被定位和表征其对防御基因表达和建立系统获得性抗性的影响。将确定受eds突变影响的病原体范围，并使用基于图谱的方法克隆EDS 4。 植物防御病原体攻击的反应尚未经过严格的遗传分析，部分原因是植物宿主已用于抗病性的分子和生物化学分析，由于大或多倍体基因组和长世代时间，植物宿主是遗传研究的困难对象。模式植物拟南芥的使用使遗传研究成为可能。因此，现在有一种强有力的方法可用于鉴定植物中尚未与已知的生物化学或分子遗传反应相关的调节因子和其他防御反应组分 *

9723493 Glazebrook Technical section Mutants of Arabidopsis thaliana with a phenotype of enhanced disease susceptibility (eds) were isolated and some were identified as alleles of NPR1 and others as alleles of PAD genes. NPR1 genes are known to be involved in the regulation of systematic acquired resistance and the PAD genes in the phytoalexin synthesis, a known defense response. In addition the EDS4 gene controls expression of the defense genes PR1 and PR5 in response to infection by a bacterial pathogen. Thus, the eds mutations define genes with important roles in plant defense against pathogen attack. The characterization of eds mutants will lead to identification of novel genes that play major roles in plant disease resistance. The EDS genes will be mapped and characterized in relation to their effect on defense gene expression and establishment of systemic acquired resistance. The range of pathogens that are affected by the eds mutations will be determined and EDS4 will be cloned using a map-based approach. Non-technical section The responses by which plants defend themselves from pathogen attack have not been subjected to rigorous genetic analysis, in part because the plant hosts have been used in molecular and biochemical analysis of disease resistance are difficult subjects for genetic studies due to large or polyploid genomes and long generation times. The use of the model plant, Arabidopsis thaliana, has made genetic studies feasible. Therefore, a powerful approach is now available for identifying regulatory factors and other defense response components in plants that are not already correlated with a known biochemical or molecular genetic response ***