Characterization of the two receptor-kinases SOBIR1/EVR and BAK1 as co-receptors of the receptor-like protein RLP30 from Arabidopsis thaliana

拟南芥受体样蛋白 RLP30 的两种受体激酶 SOBIR1/EVR 和 BAK1 的表征

• 批准号: 255705282
• 负责人: Dr. Andrea A. Gust
• 金额: --
• 依托单位: Zentrum für Molekularbiologie der Pflanzen (ZMBP)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/255705282?language=en
• 关键词: Characterization; two; receptor; kinases; SOBIR1

Plants have adapted defence mechanisms to resist microbial infection. Binding of microbial ligands to transmembrane receptors that are located at the cell surface trigger intracellular re-programming eventually leading to restriction of pathogen growth. We have associated one member of the Arabidopsis leucine-rich repeat (LRR) receptor-like proteins, RLP30, with a specific function as pattern recognition receptor for a novel elicitor activity from the fungal pathogen Sclerotinia sclerotiorum. Moreover, we have identified the two leucine-rich repeat receptor-like kinases BAK1 and SOBIR1/EVR as putative co-receptors of RLP30. The general objectives of this project will be to decipher the molecular mechanisms underlying RLP30-mediated pattern recognition and the subsequent signal transduction via the RLP30-BAK1-SOBIR1/EVR receptor machinery. Unravelling the mode of action of RLP30/BAK1/SOBIR1-mediated pattern perception will deepen our mechanistic understanding on SOBIR1/EVR as novel co-receptor for LRR-type receptor-like proteins and will broaden our knowledge on how plants adapt to a detrimental biotic environment.

植物已经适应了防御机制来抵抗微生物感染。微生物配体与位于细胞表面的跨膜受体的结合触发细胞内重编程，最终导致限制病原体生长。我们已经将拟南芥富含亮氨酸重复序列（LRR）受体样蛋白RLP 30的一个成员与真菌病原体核盘菌（Sclerotinia sclerotiorum）的一种新型激发子活性的模式识别受体的特定功能相关联。此外，我们已经确定了两个富含亮氨酸的重复序列受体样激酶BAK 1和SOBIR 1/EVR作为RLP 30的推定共受体。本项目的总体目标将是破译潜在的RLP 30介导的模式识别和随后的信号转导通过RLP 30-BAK 1-SOBIR 1/EVR受体机制的分子机制。揭示RLP 30/BAK 1/SOBIR 1介导的模式感知的作用模式将加深我们对SOBIR 1/EVR作为LRR型受体样蛋白的新型共受体的机制理解，并将拓宽我们对植物如何适应有害生物环境的知识。