RUI: Understanding Repertoire Diversification in a Nonstandard Model

RUI：理解非标准模型中的曲目多样化

• 批准号: 9723569
• 负责人: Richard Goldsby
• 金额: $ 18.45万
• 依托单位: Amherst College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9723569&HistoricalAwards=false
• 关键词: RUI; Understanding; Repertoire; Diversification; Nonstandard

9723569 Goldsby This project is studying the development of the primary antibody repertoire in cattle. In cattle the ileal Peyer's patch (IPP), a gut associated lymphoid tissue, is a major site of B cell diversification. A process of targeted, developmentally regulated somatic point mutation is responsible for the generation of diversity in the IPP. However, this is not the sole site or mechanisms for diversification in cattle, since the spleen has been shown to be a site of light chain diversification during fetal development. Also, this investigator has data suggesting that heavy chain V gene rearrangement (but not light chain) occurs in the bone marrow. Thus the following hypothesis is proposed for the development of B cells in cattle : 1)In the fetal bone marrow rearrangement of heavy but not light chain occurs; 2)Cells with rearranged heavy chain genes migrate to the spleen where light chains rearrange; 3)Peyer's patch follicles are seeded with B cells from a pool that has undergone diversification in the fetal spleen. Experiments will be done to test this hypothesis. These results will provide information on how antibody diversification develops in cattle, and will indicate species differences in the generation of diversity. This grant is from a RUI college, and undergraduate students will be involved in this research. %%% Although all higher vertebrates have similar immune systems consisting of antibodies produced by B cells and T cells which modulate the immune response, the mechanism by which organisms generate antibodies with different specificities is not the same in all species. The experiments in this project will provide information on the development of diverse antibodies in cattle. ***

小行星9723569 该项目正在研究牛一级抗体库的开发。 在牛中，回肠派伊尔集合淋巴结（IPP）是肠道相关淋巴组织，是B细胞多样化的主要部位。 有针对性的，发育调节的体细胞点突变的过程是负责在IPP的多样性的产生。 然而，这不是牛中多样化的唯一位点或机制，因为脾脏已被证明是胎儿发育期间轻链多样化的位点。 此外，该研究者有数据表明骨髓中发生重链V基因重排（而非轻链）。因此，对牛B细胞的发育提出了以下假设：1）在胎儿骨髓中发生重链而非轻链重排; 2）具有重排重链基因的细胞迁移到脾脏，在那里轻链重排; 3）派尔集合淋巴结滤泡接种有来自胎儿脾脏中经历多样化的池的B细胞。 将进行实验来检验这一假设。这些结果将提供有关牛体内抗体多样化如何发展的信息，并表明多样性产生方面的物种差异。 这项研究经费来自于RUI学院，本科生将参与这项研究。 %%% 虽然所有高等脊椎动物都有类似的免疫系统，由调节免疫反应的B细胞和T细胞产生的抗体组成，但生物体产生不同特异性抗体的机制在所有物种中并不相同。 本项目中的实验将提供牛中不同抗体的开发信息。 ***