Acquisition of a Two-Photon Microscope System for a Multi-User Facility

9724487 LEDELER，WILLIAM 该项目的目标是建立一个双光子显微镜设备，以补充和扩展巴尔的摩的马里兰州大学（UMAB）现有的多用户生物成像资源。 双光子激发避免了传统和共焦显微镜中固有的许多缺点。 例如，该方法为光解技术提供了更好的空间分辨力，并更好地渗透到厚样品中。 此外，双光子激发完全消除了远离焦点的平面中的光漂白、光损伤和背景荧光。 这些特性使双光子显微镜成为研究活细胞的一种很好的方法，并将允许UMAB研究人员完成重要的新实验。 双光子显微镜还将为学生和博士后研究员提供最先进的培训基础。

9724487 LEDERER, WILLIAM The goal of this project is to establish a two-photon microscopy facility to complement and extend existing multi- user bioimaging resources at the University of Maryland at Baltimore (UMAB). Two-photon excitation avoids many of the disadvantages inherent in conventional and confocal microscopies. For example, the approach affords better spatial discrimination for photolytic techniques and better penetration into thick samples. Furthermore, two-photon excitation entirely eliminates photobleaching, photodamage and background fluorescence in planes away from the focus. These properties make two-photon microscopy an excellent method for the study of living cells, and will permit important new experiments to be completed by UMAB researchers. A two-photon microscopy will also provide the basis for state-of-the-art training for students and postdoctoral fellows.