Genetic and Biochemical Studies of Swimming Motility in a Marine Synechococcus

海洋聚球藻游泳运动的遗传和生化研究

• 批准号: 9727759
• 负责人: Bianca Brahamsha
• 金额: $ 10万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9727759&HistoricalAwards=false
• 关键词: Genetic; Biochemical; Studies; Swimming; Motility

9727759 Brahamsha Certain marine unicellular cyanobacteria of the genus Synechococcus exhibit a mysterious type of swimming motility characterized by the absence of flagella and of any obvious organelle of motility. In previous work, Dr. Brahamsha has obtained mutants impaired in swimming motility and has identified an abundant cell-surface polypeptide, SwmA, that is required for swimming. The objectives of this project are two-fold: 1) to characterize existing motility mutants and isolate new ones and 2) to begin studies of SwmA's function. This will be accomplished by combining genetic and biochemical approaches. Studies of SwmA function will include cross-linking studies to determine the polypeptides it interacts with. Characterization of the mutants will include isolating the genes responsible, determining their nucleotide sequences, and localizing their products in Synechococcus cells. These studies should provide insights not only into the mechanism of this unique type of prokaryotic motility but also into the fundamental question of how organisms convert chemical energy into motion.

9727759 Brahamsha某些海洋单细胞蓝细菌聚球藻属表现出一种神秘的游泳运动类型，其特征是没有鞭毛和任何明显的运动细胞器。 在以前的工作中，Brahamsha博士已经获得了游泳运动受损的突变体，并确定了游泳所需的丰富的细胞表面多肽SwmA。 该项目的目标是双重的：1）表征现有的运动突变体和分离新的和2）开始研究SwmA的功能。 这将通过结合遗传和生物化学方法来实现。 SwmA功能的研究将包括交联研究，以确定与其相互作用的多肽。 突变体的表征将包括分离负责的基因，确定其核苷酸序列，并将其产物定位在聚球藻细胞中。 这些研究不仅可以深入了解这种独特的原核动力机制，还可以深入了解生物体如何将化学能转化为运动的基本问题。