RUI: Cuticular Mechanisms for Starvation Hardiness in Ticks

Adeyeye 9727813节肢动物作为陆地动物的成功部分归功于它们的角质层，这是一种充当覆盖物和骨架的多功能结构。即使在饥饿的情况下，属于鸟形动物属的扁虱也能活很多年。此外，复发性发热扁虱对干燥表现出显著的抵抗力。这个项目将调查是否以及如何将角质层0。Turicata经过生化修饰，以应对饥饿和/或干燥。饲料和饥饿的综合生化分析0。将进行斑节角质层。特别是，感兴趣的蛋白质将使用各种技术进行提纯和表征。此外，还将分析顺序饥饿的扁虱的角质层脂质。将测定角质层通透性，并确定其与角质层脂类和蛋白质变化的可能相关性。另外两种在角质层渗透性和耐饥饿性/干燥性方面不同的扁虱应该进行研究，以允许更广泛地概括角质层蛋白质和脂类在OF中的作用。抗干燥性的草莓。由于人们对硬壳的化学成分知之甚少，建议的研究将扩展有关硬壳结构和功能的现有知识。

Adeyeye 9727813 The success of arthropods as terrestrial animals is attributable partly to their cuticle, a versatile structure that acts as a covering and skeleton. Ticks belonging to the genus Ornithodoros can live for many years even when starved. Moreover, the relapsing fever tick, Ornithodoros tuncaln, shows remarkable resistance to desiccation. This project will investigate whether and how the cuticle of 0. Turicata is biochemically modified in order to cope with starvation and/or desiccation. Comprehensive biochemical analyses of fed and starved 0. turicata cuticle will be conducted. In particular, proteins of interest will be purified and characterized using various techniques. Also cuticular lipids of sequentially starved ticks will be analyzed. The cuticular permeability will be determined and its possible correlation with changes in cuticular lipids and proteins will be ascertained. Two other ticks that differ in cuticular permeability and in starvation/desiccation hardiness shall be studied to permit broader generalization about the role of cuticular proteins and lipids in the of 0. turicata resisting desiccation. Because so little is known about the chemistry of the cuticle of soft ticks, the proposed studies will expand existing knowledge vis-a-vis the structure and functions of tick cuticle.