Characterization of Higher Plant Phosphoinositide-specific Phospholipase C

高等植物磷酸肌醇特异性磷脂酶 C 的表征

• 批准号: 9728024
• 负责人: Wolf-Dieter Reiter
• 金额: $ 11万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728024&HistoricalAwards=false
• 关键词: Characterization; Higher; Plant; Phosphoinositide; specific

Abstract 9728024 Crain The biochemical mechanisms by which plants perceive and respond to changes in the environment are poorly understood. This investigator believes that the breakdown of phosphoinositide lipids brings environmental information into the plant cell. Phosphoinositide breakdown produces inositol phosphates and diacylglycerol, which play key roles in regulating cell physiology. Phosphoinositide specific phospholipases C (PLC) are critical in the transduction of hormonal and environmental signals in animal cells. Plant cells also contain PLCs specific for phosphoinositides, and there is evidence that these enzymes are involved in mediating plant cell response to environmental stimuli. Although some of these enzymes have been partially purified and some cloned, little is known about the properties or regulation of these enzymes, or how many PLCs are present in plant cells. The experiments described here will investigate this class of enzymes in order to understand their regulation and role in plant responses to environmental signals. The enzymes will be identified in oat roots and purified, sequenced and cloned, and their regulation examined. The research described here will identify, purify and study the regulation of phospholipase C enzymes in plants, which breakdown lipids known as phosphoinositides, and which are thought to play an important role in the perception of and response to environmental stimuli. ***

摘要9728024克莱恩 植物感知和响应环境变化的生化机制知之甚少。 这位研究者认为，磷脂酰肌醇脂质的分解将环境信息带入植物细胞。 磷酸肌醇分解产生肌醇磷酸和甘油二酯，它们在调节细胞生理学中起关键作用。 磷脂酰肌醇特异性磷脂酶C（PLC）在动物细胞内激素和环境信号的转导中起着关键作用。 植物细胞还含有对磷酸肌醇特异的PLC，并且有证据表明这些酶参与介导植物细胞对环境刺激的反应。 虽然这些酶中的一些已经被部分纯化和克隆，但对这些酶的性质或调节，或者植物细胞中存在多少PLC知之甚少。 这里描述的实验将研究这类酶，以了解它们在植物对环境信号的反应中的调节和作用。 这些酶将在燕麦根中鉴定，纯化，测序和克隆，并检查它们的调节。 这里描述的研究将识别，纯化和研究植物中磷脂酶C酶的调节，该酶分解称为磷酸肌醇的脂质，并被认为在感知和响应环境刺激中发挥重要作用。 ***