Lipid Metabolism in the Oyster Protozoan Parasite, PERKINSUS MARINUS

牡蛎原生动物寄生虫 PERKINSUS MARINUS 的脂质代谢

• 批准号: 9728284
• 负责人: Fu-Lin Chu
• 金额: $ 24.57万
• 依托单位: College of William & Mary Virginia Institute of Marine Science
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728284&HistoricalAwards=false
• 关键词: Lipid; Metabolism; Oyster; Protozoan; Parasite

9728284 Chu The protozoan parasite Perkinsus marinus (Dermo) is one of the two important parasites that have been causing severe mortality in the American (eastern) oyster, Crassostrea virginica, on the eastern and Gulf coasts of the United States since the 1950's. Presently, no information exists on lipid metabolism and biosynthesis in this parasite, although its host is an ecologically and economically important aquatic species. Using radiolabeled or "fluorescent-labeled" precursors, the investigator will characterize lipid metabolism and biosynthesis in this parasite in relation to its growth phases and life stages. She will also determine if temperature and salinity affect the parasite's lipid metabolism and biosynthesis and, in addition, will investigate the effect of P. marinus parasitism on the lipid metabolism of the host. She will use gas liquid chromatography (GLC), thin layer chromatography/flame ionization detector (TLC/FID) lipid analyzer (Iatroscan MK-3), high performance TLC, argentation TLC, and high pressure liquid chromatography (HPLC) equipped with a flow scintillation analyzer to analyze the uptake/incorporation, distribution, metabolism and synthesis of lipids, particularly phospholipids, and fatty acids in the parasite. The identification of fatty acids will be confirmed with an ELQ400-2 GC/MS system. The morphological distribution of "fluorescent labels" will be examined by epifluorescent and electron microscopy. Parasites are the major cause of disease in oysters on the eastern and Gulf coasts of the United States. These parasites derive their food, mainly in the form of lipids and protein, from the oyster. This is the first study of lipid metabolism of an internal parasite of an aquatic invertebrate species. The results from this research will contribute to the fundamental understanding of lipid metabolism in host-parasite relationships, especially in saltwater systems. The study will provide important information for the establishment of a host-parasite in vertebrate model to elucidate mechanisms involved in lipid acquisition in parasites. This information may also serve as the basis for the future development of a procedure to control oyster parasites.

9728284 Chu原生动物寄生虫Perkinsus marinus（Dermo）是自20世纪50年代以来在美国东部和海湾海岸的美洲（东部）牡蛎Crassostrea virginica中引起严重死亡的两种重要寄生虫之一。 目前，没有信息存在于这种寄生虫的脂质代谢和生物合成，虽然它的主机是一个生态和经济上重要的水生物种。使用放射性标记或“荧光标记”的前体，研究人员将描述这种寄生虫与其生长阶段和生命阶段有关的脂质代谢和生物合成。她还将确定温度和盐度是否会影响寄生虫的脂质代谢和生物合成，此外，她还将研究P. marinus寄生对宿主脂质代谢的影响。 她将使用气液色谱法（GLC）、薄层色谱法/火焰离子化检测器（TLC/FID）脂质分析仪（Iatroscan MK-3）、高效TLC、argentation TLC和配备流动闪烁分析仪的高压液相色谱法（HPLC）分析寄生虫中脂质（特别是磷脂）和脂肪酸的摄取/掺入、分布、代谢和合成。 将使用ELQ 400 -2 GC/MS系统确认脂肪酸的鉴别。 将通过落射荧光显微镜和电子显微镜检查“荧光标记”的形态分布。 寄生虫是美国东部和海湾沿岸牡蛎疾病的主要原因。 这些寄生虫从牡蛎中获取食物，主要是脂质和蛋白质。 这是第一个研究的内部寄生虫的水生无脊椎动物物种的脂质代谢。 这项研究的结果将有助于从根本上了解宿主-寄生虫关系中的脂质代谢，特别是在盐水系统中。 本研究为建立宿主-寄生虫动物模型，阐明寄生虫脂质获取的机制提供了重要信息。 这些信息也可以作为未来开发控制牡蛎寄生虫程序的基础。