Integrated Rapid Mixing Apparatus for CD, Fluorescence, Absorbance, Raman, Chemical Quench and Freeze Quench

用于 CD、荧光、吸光度、拉曼、化学淬灭和冷冻淬灭的集成快速混合装置

• 批准号: 9729366
• 负责人: Denis Rousseau
• 金额: --
• 依托单位: Yeshiva University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9729366&HistoricalAwards=false
• 关键词: Integrated; Rapid; Mixing; Apparatus; CD

This proposal will provide funds for an integrated rapid mixing system that will allow stopped flow, continuous flow, freeze quench and chemical quench experiments to be carried out. In the stopped or continuous flow modes reactions can be studied by absorption, fluorescence or Raman scattering and circular dichroism. In the chemical and freeze quench modes reactive intermediates can be trapped and then characterized by electron paramagnetic resonance, nuclear magnetic resonance, or other spectroscopies. Projects will be conducted by six major users from the Departments of Biochemistry and Physiology and Biophysics. The instrumentation will also be available to several additional users and will be part of a NIH supported Training Program in Molecular Biophysics. In general, the instrumentation will be applied to the study of protein and ribozyme folding, several enzyme substrate interactions, ligand binding in hemoglobins, protein-DNA interactions and protein translocation.

该提案将为一个集成的快速混合系统提供资金，该系统将允许进行停流、连续流、冷冻淬火和化学淬火实验。 在停止或连续流动模式下，可以通过吸收、荧光或拉曼散射和圆二色性来研究反应。 在化学和冷冻淬灭模式下，可以捕获活性中间体，然后通过电子顺磁共振、核磁共振或其他光谱进行表征。 项目将由来自生物化学和生理学以及生物物理学系的六个主要用户进行。 该仪器还将提供给其他几个用户，并将成为NIH支持的分子生物物理学培训计划的一部分。 在一般情况下，仪器将被应用于蛋白质和核酶折叠，几种酶底物相互作用，血红蛋白中的配体结合，蛋白质-DNA相互作用和蛋白质易位的研究。