Development and Application of Oligonucleotide Synthesis Methods

拟议的研究涉及合成新的“正交”固体 阶段支持。这些将用于制备受保护的 寡核苷酸和具有官能团的寡核苷酸缀合物 或连接在确定位置的肽。 相关寡核苷酸 也将准备。 这项研究将扩大反应的类型， 其可用于从固体支持物上切割寡核苷酸， 包括还原反应和酶促反应，并扩展了 可用于缀合受保护的寡核苷酸的化学方法。 有了这个更新奖，有机和高分子化学计划 支持Marc M.博士的研究。格林伯格的部门 化学在科罗拉多州立大学。 重点研究 开发固相支持物，从其保护DNA片段， RNA可以在温和的条件下被切割， 的反应可用于延长连接到 形成低聚物。 这项工作将为其他人提供有用的方法 尝试合成寡核苷酸，并将有实际的 生物医学应用 研究生和博士后研究员 将接受小分子合成的培训， 生物聚合物，并获得生长所需的培训， 生物技术领域。

The proposed research involves the synthesis of novel `orthogonal` solid phase supports. These will be used for the preparation of protected oligonucleotides, and oligonucleotide conjugates with functional groups or peptides attached at defined positions. Related oligoribonucleotides will also be prepared. The research will extend the types of reactions that can be used to cleave the oligonucleotides from the solid support to include reductive and enzymatic reactions, and extend the scope of chemistry available for conjugating protected oligonucleotides. With this renewal award, the Organic and Macromolecular Chemistry Program supports the research of Dr. Marc M. Greenberg of the Department of Chemistry at Colorado State University. The research focuses on developing solid phase supports from which protected fragments of DNA and RNA can be cleaved under mild conditions, and on expanding the repertoire of reactions available for extending the number of nucleotides linked to form the oligomers. The work will provide useful approaches to others attempting to synthesize oligonucleotides, and will have practical biomedical applications. Graduate students and post-doctoral fellows will receive training in the synthesis of small molecules and biopolymers, and get the kind of training needed in the growing biotechnology area.