Development and Application of Solid Phase Oligonucleotide Synthesis Supports

通过这一奖项，合成有机计划和多学科活动办公室支持科罗拉多州立大学化学系马克·M·格林伯格博士的研究。格林伯格博士将把工作重点放在开发固相寡核苷酸合成载体上。由此得到的受保护的寡核苷酸具有重要的生物学特性，这项工作具有重要的生物技术意义。这项研究的重点是开发用于固相寡核苷酸合成的正交连接物，利用已知的光化学反应和有机金属反应，以及从这些新型载体释放的生物聚合物的偶联。结合方法学将侧重于寡核苷酸和多肽之间以及两个寡核苷酸之间的键的形成。该方法将对合成RNA分子特别有用。

With this award, the Synthetic Organic Program and the Office of multidisciplinary Activities are supporting the research of Dr. Marc M. Greenberg of the Department of Chemistry at Colorado State University. Dr. Greenberg will focus his work on developing solid phase oligonucleotide synthesis supports. The resulting protected oligonucleotides have important biological properties and the work has important biotechnical implications. The focus of this research is the development of orthogonal linkers for solid phase oligonucleotide synthesis that utilize known photochemical and organometallic reactions, and conjugation of the biopolymers released from these novel supports. Conjugation methodology will focus on the formation of bonds between oligonucleotides and peptides as well as between two oligonucleotides. The methodology will be particularly useful for the synthesis of RNA molecules.