CAREER: Immuno-Optical Biosensors for Plasma Factors/ Application of TQM in Bioengineering Education

职业：血浆因子免疫光学生物传感器/全面质量管理在生物工程教育中的应用

• 批准号: 9733207
• 负责人: Kyung Kang
• 金额: $ 31万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-15 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9733207&HistoricalAwards=false
• 关键词: CAREER; Immuno; Optical; Biosensors; Plasma

9733207 Kang Protein C (PC) is an important blood plasma protein which prevents unwanted clots and also dissolves formed clots in the human body. With PC deficiency, either by hereditary or acquired, the human body can encounter serious medical complications due to blood coagulation that can lead to embolisms that injure lung, heart, and brain tissue, and can cause death. A practical, clinical PC biosensor for the diagnosis of PC deficiency should have the attributes of high sensitivity, high specificity, and real-time measurement. The proposed mechanism depends on a highly specific antigen-antibody reaction. It involves binding antibodies at the surface of an optical fiber, injection of the sample into a micro chamber (300 micro liter) encasing the fiber, reacting light emitting secondary antibodies with the absorbed PC molecules on the fiber, and quantifying the PC concentration by the emitted light intensity. It is proposed to apply total quality management (TQM) concepts in both graduate and undergraduate bioengineering education and research. This project emphasizes the structured team approach to bring together basic scientists, bioengineers (biomedical and bioprocess engineers), clinicians, business, and legal professionals to address optimum process development for the production of the best bioproduct at minimum cost. ***

9733207康蛋白C（PC）是一种重要的血浆蛋白，可防止不需要的凝块，并溶解人体内形成的凝块。 由于PC缺乏症，无论是遗传性的还是获得性的，人体都可能因血液凝固而遇到严重的医疗并发症，这可能导致损伤肺，心脏和脑组织的栓塞，并可能导致死亡。 一个实用的，临床PC生物传感器的PC缺陷的诊断应具有高灵敏度，高特异性，和实时测量的属性。 所提出的机制取决于高度特异性的抗原-抗体反应。 它涉及在光纤表面结合抗体，将样品注入包裹光纤的微室（300微升），使发光二抗与光纤上吸收的PC分子反应，并通过发射的光强度定量PC浓度。 建议在研究生和本科生生物工程教育和研究中应用全面质量管理（TQM）概念。 该项目强调结构化的团队方法，将基础科学家，生物工程师（生物医学和生物工艺工程师），临床医生，商业和法律的专业人士聚集在一起，以解决以最低成本生产最佳生物产品的最佳工艺开发。 ***