CAREER: Immuno-Optical Biosensors for Plasma Factors/ Application of TQM in Bioengineering Education

康蛋白C （PC）是一种重要的血浆蛋白，可以防止不需要的凝块，也可以溶解人体内形成的凝块。PC缺乏症，无论是遗传性的还是后天的，人体都会遇到严重的医疗并发症，因为血液凝固会导致栓塞，损伤肺、心脏和脑组织，并可能导致死亡。一种实用的临床诊断PC缺乏的PC生物传感器应具有高灵敏度、高特异性和实时测量的特点。提出的机制依赖于高度特异性的抗原-抗体反应。它包括在光纤表面结合抗体，将样品注射到包裹光纤的微室（300微升）中，与光纤上吸收的PC分子发生发光的二抗反应，并通过发射的光强度来定量PC浓度。建议将全面质量管理（TQM）概念应用于研究生和本科生的生物工程教育和研究。该项目强调结构化的团队方法，将基础科学家、生物工程师（生物医学和生物工艺工程师）、临床医生、商业和法律专业人员聚集在一起，以最小的成本解决生产最佳生物产品的最佳工艺开发问题。＊＊＊

9733207 Kang Protein C (PC) is an important blood plasma protein which prevents unwanted clots and also dissolves formed clots in the human body. With PC deficiency, either by hereditary or acquired, the human body can encounter serious medical complications due to blood coagulation that can lead to embolisms that injure lung, heart, and brain tissue, and can cause death. A practical, clinical PC biosensor for the diagnosis of PC deficiency should have the attributes of high sensitivity, high specificity, and real-time measurement. The proposed mechanism depends on a highly specific antigen-antibody reaction. It involves binding antibodies at the surface of an optical fiber, injection of the sample into a micro chamber (300 micro liter) encasing the fiber, reacting light emitting secondary antibodies with the absorbed PC molecules on the fiber, and quantifying the PC concentration by the emitted light intensity. It is proposed to apply total quality management (TQM) concepts in both graduate and undergraduate bioengineering education and research. This project emphasizes the structured team approach to bring together basic scientists, bioengineers (biomedical and bioprocess engineers), clinicians, business, and legal professionals to address optimum process development for the production of the best bioproduct at minimum cost. ***