SENSORS: Rapid, Multi-Analyte Immuno Biosensor with Passive Microfluidics: A Model System - Four Cardiac Marker Monitoring Device

0330075 Kang本研究的目标是开发抗体敏化的锥形光纤传感器阵列和寡核苷酸签名光纤传感器，用于快速直接检测和量化生物恐怖分子及其DNA签名，具有高特异性和灵敏度，并具有极低的假阳性。所提出的传感器将利用连续双锥形光纤中的消逝吸收、散射和荧光来进行检测和定量。研究人员将检查用于检测的单光子和多光子消逝吸收、散射和荧光。所提出的传感器不仅将以非常高的灵敏度测量生物恐怖主义制剂(S)的存在，而且还将确定它们的生存能力。多种策略的使用：在单光子和多光子模式下的消逝吸收、散射和荧光，以及表面固定的单抗和针对目标生物体的独特DNA序列的辅助下，提供了一种全面的检测方法。这项研究的更广泛影响是多方面的。检测和识别病原体或生物恐怖主义制剂的存在或不存在的能力对打击恐怖主义至关重要。此外，指出病原体的性质，特别是其生存能力和毒力是至关重要的。如果这些都能在一种细菌的分解下迅速完成，对社会的好处可能是巨大的。此外，根据这项研究开发的传感器还可以用于体内组织的代谢成像。通过将锥体定位在组织外部，双光子荧光激发的穿透将使几厘米深处的代谢活动信息能够被光学测定。乳房上方的这些锥形序列(例如)可以识别表面下几厘米处的恶性肿瘤。

0330075KangThe goal of this research is to develop Antibody Sensitized Tapered Fiber Sensors arrays and Oligonucleotide Signatured Fiber Sensors for rapid direct detection and quantification of bioterrorism agents and their confirming DNA signatures using optical means with high specificity and sensitivity, and very low false positives. The proposed sensors will use evanescent absorption, scatter and fluorescence in continuous biconical tapered fibers for detection and quantification. The investigators will examine one- and multi- photon evanescent absorption, scatter and fluorescence for detection. The proposed sensors will not only measure the presence of bioterrorism agent(s) at very high sensitivity, but also establish their viability. The use of multiple strategies: evanescent absorption, scatter and fluorescence in single and multi-photon mode with the aid of surface immobilized monoclonal antibodies and unique DNA sequences specific to the target organism offers a comprehensive approach for detection. The broader impacts of this research are several-fold. The ability to detect and identify the presence or absence of pathogens or bioterrorism agents is essential for fighting terrorism. In addition, pin pointing the nature of the pathogen, especially its viability and virulence is paramount. If these can be accomplished expeditiously at the resolution of a single bacterium, the benefits to the society could be enormous. In addition, the sensors developed under this research can also be used for metabolic imaging of tissue in vivo. By positioning the tapers external to the tissue, the penetration of the 2-photon fluorescence excitation will enable the metabolic activity information from depths of several centimeters to be determined optically. A sequence of these tapers over the breast (as an example) can identify malignant tumors several centimeters beneath the surface.