SENSORS: Rapid, Multi-Analyte Immuno Biosensor with Passive Microfluidics: A Model System - Four Cardiac Marker Monitoring Device

传感器：具有被动微流体的快速多分析物免疫生物传感器：模型系统 - 四心脏标记物监测装置

• 批准号: 0330075
• 负责人: Kyung Kang
• 金额: $ 68.6万
• 依托单位: University of Louisville Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0330075&HistoricalAwards=false
• 关键词: SENSORS; Rapid; Multi; Analyte; Immuno

0330075KangThe goal of this research is to develop Antibody Sensitized Tapered Fiber Sensors arrays and Oligonucleotide Signatured Fiber Sensors for rapid direct detection and quantification of bioterrorism agents and their confirming DNA signatures using optical means with high specificity and sensitivity, and very low false positives. The proposed sensors will use evanescent absorption, scatter and fluorescence in continuous biconical tapered fibers for detection and quantification. The investigators will examine one- and multi- photon evanescent absorption, scatter and fluorescence for detection. The proposed sensors will not only measure the presence of bioterrorism agent(s) at very high sensitivity, but also establish their viability. The use of multiple strategies: evanescent absorption, scatter and fluorescence in single and multi-photon mode with the aid of surface immobilized monoclonal antibodies and unique DNA sequences specific to the target organism offers a comprehensive approach for detection. The broader impacts of this research are several-fold. The ability to detect and identify the presence or absence of pathogens or bioterrorism agents is essential for fighting terrorism. In addition, pin pointing the nature of the pathogen, especially its viability and virulence is paramount. If these can be accomplished expeditiously at the resolution of a single bacterium, the benefits to the society could be enormous. In addition, the sensors developed under this research can also be used for metabolic imaging of tissue in vivo. By positioning the tapers external to the tissue, the penetration of the 2-photon fluorescence excitation will enable the metabolic activity information from depths of several centimeters to be determined optically. A sequence of these tapers over the breast (as an example) can identify malignant tumors several centimeters beneath the surface.

0330075Kang这项研究的目标是开发抗体敏化锥形光纤传感器阵列和寡核苷酸签名光纤传感器，用于快速直接检测和量化生物恐怖分子及其使用具有高特异性和灵敏度以及极低误报的光学手段确认DNA签名。 所提出的传感器将利用连续双锥形光纤中的倏逝吸收、散射和荧光来进行检测和定量。 研究人员将检查单光子和多光子倏逝吸收、散射和荧光以进行检测。 所提出的传感器不仅能够以非常高的灵敏度测量生物恐怖主义制剂的存在，而且还能确定它们的生存能力。 多种策略的使用：单光子和多光子模式下的瞬逝吸收、散射和荧光，借助表面固定的单克隆抗体和目标生物体特有的独特 DNA 序列，提供了一种全面的检测方法。 这项研究的更广泛影响是多方面的。 检测和识别病原体或生物恐怖主义制剂是否存在的能力对于打击恐怖主义至关重要。 此外，查明病原体的性质，尤其是其活力和毒力至关重要。如果这些可以在单个细菌的分辨率下迅速完成，那么对社会的好处将是巨大的。 此外，本研究开发的传感器还可用于体内组织的代谢成像。 通过将锥体定位在组织外部，2 光子荧光激发的穿透将使几厘米深度的代谢活动信息能够通过光学方式确定。 乳房上的一系列锥形（例如）可以识别表面以下几厘米的恶性肿瘤。