SENSORS: Rapid, Multi-Analyte Immuno Biosensor with Passive Microfluidics: A Model System - Four Cardiac Marker Monitoring Device

本研究的目标是开发抗体敏化锥形光纤传感器阵列和寡核苷酸签名光纤传感器，用于生物恐怖主义制剂的快速直接检测和定量，并使用光学手段确认其DNA特征，具有高特异性和灵敏度，假阳性率极低。该传感器将利用连续双锥形锥形光纤中的倏逝吸收、散射和荧光进行检测和定量。研究人员将检测单光子和多光子的倏逝吸收、散射和荧光。所提出的传感器不仅可以非常高的灵敏度测量生物恐怖制剂的存在，而且还可以确定它们的生存能力。利用多种策略：在单光子和多光子模式下的瞬时吸收、散射和荧光，借助于表面固定的单克隆抗体和目标生物特异性的独特DNA序列，提供了一种全面的检测方法。这项研究的广泛影响是多方面的。探测和识别病原体或生物恐怖主义制剂是否存在的能力对于打击恐怖主义至关重要。此外，确定病原体的性质，特别是其生存能力和毒力是至关重要的。如果这些能够在单个细菌的分辨率上迅速完成，对社会的好处将是巨大的。此外，本研究开发的传感器还可用于体内组织的代谢成像。通过将锥体放置在组织外部，双光子荧光激发的穿透将使从几厘米深处的代谢活动信息能够被光学确定。在乳房上的一系列这些锥体（例如）可以识别表面下几厘米的恶性肿瘤。

0330075KangThe goal of this research is to develop Antibody Sensitized Tapered Fiber Sensors arrays and Oligonucleotide Signatured Fiber Sensors for rapid direct detection and quantification of bioterrorism agents and their confirming DNA signatures using optical means with high specificity and sensitivity, and very low false positives. The proposed sensors will use evanescent absorption, scatter and fluorescence in continuous biconical tapered fibers for detection and quantification. The investigators will examine one- and multi- photon evanescent absorption, scatter and fluorescence for detection. The proposed sensors will not only measure the presence of bioterrorism agent(s) at very high sensitivity, but also establish their viability. The use of multiple strategies: evanescent absorption, scatter and fluorescence in single and multi-photon mode with the aid of surface immobilized monoclonal antibodies and unique DNA sequences specific to the target organism offers a comprehensive approach for detection. The broader impacts of this research are several-fold. The ability to detect and identify the presence or absence of pathogens or bioterrorism agents is essential for fighting terrorism. In addition, pin pointing the nature of the pathogen, especially its viability and virulence is paramount. If these can be accomplished expeditiously at the resolution of a single bacterium, the benefits to the society could be enormous. In addition, the sensors developed under this research can also be used for metabolic imaging of tissue in vivo. By positioning the tapers external to the tissue, the penetration of the 2-photon fluorescence excitation will enable the metabolic activity information from depths of several centimeters to be determined optically. A sequence of these tapers over the breast (as an example) can identify malignant tumors several centimeters beneath the surface.