CAREER: The Role of Vesicular Transport on the Function and Biogenesis of the Contractile Vacuole in Dictyostelium Discoideum

职业：盘基网柄菌中囊泡运输对收缩液泡功能和生物发生的作用

• 批准号: 9734093
• 负责人: John Bush
• 金额: --
• 依托单位: University of Arkansas Little Rock
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 2005-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9734093&HistoricalAwards=false
• 关键词: CAREER; Role; Vesicular; Transport; Function

9734093 Bush Abstract The contractile vacuole has long been studied as a subcellular model for the mammalian kidney. In Protozoans and other primitive animals where it is found, it is an essential organelle that maintains water and ion homeostasis within the organism. Presumably, study of its structure and function will provide insights into how more complex systems have evolved to perform these same functions in higher animals. This proposal exploits the genetics of Dictyostelium discoideum to study the role of a particular GTPase (Rab11) in the biogenesis of the contractile vacuole. Previous studies have shown that this protein is localized to the contractile vacuole and, arguing from studies of Rab function in other systems, is probably important in the regulation of vesicle traffic to and from this compartment. The studies will utilize a number of genetic approaches to study how normal and mutant versions of Rab11 influence the biogenesis of the vacuole. Additional studies will evaluate the possibility that other Rab-associated proteins are important in Rab control of vacuole biogenesis and function. ***

9734093布什摘要长期以来，收缩空泡一直被作为哺乳动物肾脏的亚细胞模型进行研究。在原生动物和其他发现它的原始动物中，它是维持有机体内水和离子动态平衡的重要细胞器。大概，对它的结构和功能的研究将为更复杂的系统如何进化到在高等动物身上执行同样的功能提供洞察力。这一建议利用盘基网柄菌的遗传学来研究一种特定的GTP酶(Rab11)在收缩液泡的生物发生中的作用。以前的研究表明，这种蛋白定位于收缩的液泡，从其他系统中对RAB功能的研究来看，它可能在调节囊泡进出这个隔室的运输中起着重要作用。这些研究将利用一些遗传方法来研究Rab11的正常和突变版本如何影响液泡的生物发生。其他研究将评估其他Rab相关蛋白在Rab控制液泡生物发生和功能中的重要作用的可能性。***