SBIR Phase I: Method for Isolating and Assaying Marine Microorganisms

*** 9761407 Akselband这个小型企业创新研究（SBIR）项目涉及海洋微生物分离物的新型分离和培养方法，以利用海洋环境的巨大多样性。迄今为止，由于分离和培养生产生物的困难，很少有研究针对海洋细菌的天然产物。据估计，使用标准方法可以培养和分离的海洋细菌不到5%。第一阶段将开发一种分离、培养和分析海洋微生物活性物质的方法。单细胞封装将与高通量筛选方法相结合，以允许分析用于治疗和工业产品的物质的小细胞亚群。这项技术将允许克隆用其他方法无法分离的生长缓慢的生物体。新的分离方法的发展有望加强海洋和陆地样品的快速自动化天然产物筛选。海洋生物是新型生物活性代谢物的巨大潜在来源。改进的分离、培养和筛选方法将允许对有用的治疗和工业产品进行商业筛选。* * *

*** 9761407 Akselband This Small Business Innovation Research (SBIR) project addresses novel isolation and culture methods for marine microbial isolates in order to take advantage of the vast diversity of ocean environments. To date, little study has been directed toward natural products from marine bacteria due to difficulties isolating and culturing producing organisms. It is estimated that fewer than 5% of marine bacteria can be cultured and isolated using standard methods. Phase I will develop a method for isolating, culturing, and assaying marine microorganisms for active substances. Single cell encapsulation will be combined with high-throughput screening methods to allow analysis of small subpopulations of cells producing substances for therapeutic and industrial products. This technique will allow cloning of slow growing organisms that can not be isolated using other methods. Development of new isolation methods is expected to enhance rapid automated natural product screening of marine as well as terrestrial samples. Marine organisms represent a vast potential source of novel bioactive metabolites. Improved isolation, culturing, and screening methods will permit commercial screening for useful therapeutic and industrial products. ***