The Role of Structural Intermediates in Protein Folding

结构中间体在蛋白质折叠中的作用

• 批准号: 9803535
• 负责人: Wilfredo Colon
• 金额: $ 3.5万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9803535&HistoricalAwards=false
• 关键词: Role; Structural; Intermediates; Protein; Folding

9803535 Colon This study is to investigate how proteins fold, using a histidine-containing protein from E. coli as a model system since its three-dimensional structure is known. Experiments are designed to determine if kinetic intermediates can be detected during the processes of folding and unfolding, and whether basic principles can be derived from such information. The strategy will rely on the application of stopped-flow methods combined with optical spectroscopy and site-directed mutagenesis. The results should be fundamental to the understanding of protein structure and design.

9803535 结肠 这项研究旨在研究蛋白质如何折叠，使用来自大肠杆菌的含组氨酸蛋白质作为模型系统，因为其三维结构已知。实验旨在确定是否可以在折叠和展开过程中检测到动力学中间体，以及是否可以从这些信息中推导出基本原理。 该策略将依赖于停流方法与光谱学和定点诱变相结合的应用。 结果对于理解蛋白质结构和设计应该是基础。