Role of mast cells in innate and adaptive immune response

肥大细胞在先天性和适应性免疫反应中的作用

• 批准号: 125480101
• 负责人: Professorin Dr. Anne Dudeck
• 金额: --
• 依托单位: Institut für Immunologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/125480101?language=en
• 关键词: Role; mast; cells; innate; adaptive

Mast cells are well known as mediators of potentially life-threatening anaphylaxis. Beneficial functions of these cells are far less understood. Most of current textbook knowledge on mast cell in vivo functions stems from experiments in mast cell-deficient kit mutant mice as models of mast cell-deficiency and their reconstitution with in vitro-differentiated mast cells. Using novel Cre/loxP-based (and kit independent) models of mast cell-deficiency and mast cellspecific gene inactivation, which we recently generated, we found that mast cells are essential promoters of the T cell response to organic haptens. This finding is at conflict with published literature reporting, based on experiments in kit mutant mice, that mast cells suppress these responses. We conclude that results obtained in kit mutant mice, even if validated by mast cell reconstitution, do not necessarily reflect the situation in normal mice. Therefore, we consider the important question whether mast cells play relevant roles in innate immunity as well as T cell and humoral responses still open. The aim of the project is to re-address the contribution of mast cells in classical models of innate defense against bacterial pathogens and adaptive immune responses.

众所周知，肥大细胞是潜在危及生命的过敏反应的介质。这些细胞的有益功能还远未被了解。目前大多数关于肥大细胞体内功能的教科书知识源于在肥大细胞缺陷kit突变小鼠中作为肥大细胞缺陷模型的实验以及它们与体外分化的肥大细胞的重建。使用新的Cre/loxP为基础的（和试剂盒独立）模型的肥大细胞缺陷和肥大细胞特异性基因失活，我们最近产生的，我们发现，肥大细胞是必不可少的启动子的T细胞响应有机半抗原。这一发现与发表的文献报道相冲突，该文献报道基于kit突变小鼠的实验，肥大细胞抑制这些反应。我们的结论是，在试剂盒突变小鼠中获得的结果，即使通过肥大细胞重建验证，也不一定反映正常小鼠的情况。因此，我们认为肥大细胞是否在先天免疫以及T细胞和体液反应中发挥相关作用的重要问题仍然是开放的。该项目的目的是重新解决肥大细胞在针对细菌病原体的先天防御和适应性免疫反应的经典模型中的贡献。

项目成果

Engulfment of mast cell secretory granules on skin inflammation boosts dendritic cell migration and priming efficiency

• DOI: 10.1016/j.jaci.2018.08.052
• 发表时间: 2019-05-01
• 期刊: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
• 影响因子: 14.2
• 作者: Dudeck, Jan;Froebel, Julia;Dudeck, Anne
• 通讯作者: Dudeck, Anne

Mast cells acquire MHCII from dendritic cells during skin inflammation

• DOI: 10.1084/jem.20160783
• 发表时间: 2017-12-01
• 期刊: JOURNAL OF EXPERIMENTAL MEDICINE
• 影响因子: 15.3
• 作者: Dudeck, Jan;Medyukhina, Anna;Dudeck, Anne
• 通讯作者: Dudeck, Anne