Vascularization and loading of amino acids into nematode-induced feeding sites

血管化和氨基酸加载到线虫诱导的摄食位点

• 批准号: 125807889
• 负责人: Privatdozent Dr. Ulrich Hammes
• 金额: --
• 依托单位: Lehrstuhl für Systembiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/125807889?language=en
• 关键词: Vascularization; loading; amino; acids; into

Two different groups of plant-parasitic sedentary nematodes, the cyst nematodes and the root-knot nematodes, withdraw nutrients from their host’s roots from highly specialized cellular feeding structures. In the case of cyst nematodes nutrients enter the so-called syncytium symplastically, via plasmodesmata. In the case of root-knot nematodes the feeding structure consist of giant cells which are symplastically isolated and, therefore, depend on transport proteins for nutrient import. Both feeding sites are connected to a highly specialized type of unloading phloem, which, in case of root-knot nematodes consist exclusively of sieve elements. The mechanisms that control vascularization of feeding sites and transport proteins that play a role in nutrient import into giant cells are largely unknown. One aim of this proposal is to elucidate the events that control vascularization of feeding sites. Particularly the role of the phytohormone auxin will be addressed. Furthermore the role of a sieve element marker and the role of plasmodesmata localized receptors will be investigated. The other part of the proposal aims to characterize the role of an amino acid transporter in sink tissues, especially in giant cells. The changes of its localization in response to nutrient status and nematode infestation will be investigated.

两种不同的植物寄生的定居线虫，包囊线虫和根结线虫，从高度专门化的细胞喂养结构的宿主的根部提取营养物质。在胞囊线虫的情况下，营养物质通过胞间连丝共质体进入所谓的合胞体。在根结线虫的情况下，摄食结构由共质体分离的巨细胞组成，因此，依赖于营养输入的转运蛋白。这两个喂养网站连接到一个高度专业化的类型卸载韧皮部，其中，在根瘤线虫的情况下，完全由筛元件。控制摄食部位血管化的机制和在营养输入到巨细胞中起作用的转运蛋白在很大程度上是未知的。这个提议的一个目的是阐明控制摄食部位血管化的事件。特别是植物激素生长素的作用将得到解决。此外，筛元件标记物的作用和胞间连丝定位受体的作用将被研究。该提案的另一部分旨在描述氨基酸转运蛋白在水槽组织中的作用，特别是在巨细胞中。其定位的变化，响应营养状况和线虫侵染将进行调查。