POWRE: Identification of Auxin Signal Transduction Mutants in Arabidopsis

POWRE：拟南芥中生长素信号转导突变体的鉴定

• 批准号: 9805787
• 负责人: Jane Murfett
• 金额: $ 7.5万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-15 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9805787&HistoricalAwards=false
• 关键词: POWRE; Identification; Auxin; Signal; Transduction

9805787 Murfett This POWRE proposal will investigate the auxin signal transduction pathway using a novel and well developed genetic approach. Auxin, which is an essential plant growth hormone, has been the subject of intensive research for over 100 years. However, the mechanisms by which this essential growth substance functions remain unknown. The proposed research will almost certainly provide important insights for understanding auxin action. The work utilizes a number of novel transgenic plant lines that the PI has constructed to use a reporter gene that is under the control of an auxin-specific promoter. The transgenic plants were mutagenized and mutant lines that display altered expression of the reporter gene were isolated. Several novel mutants have already been isolated and the proposed research will result in detailed characterization the mutant plants and eventually cloning of the genes. Because of the central role of auxin in plant growth and development, identifying the genes involved in the transduction of auxin signals will be of fundamental significance to plant biology research and may eventually result in novel methods for controlling crop production . The proposed research will also allow the PI to develop an excellent long-term research project that will demonstrate her ability to be an independent researcher and make her very competitive when searching for permanent faculty positions.

这项POWRE提案将使用一种新颖而成熟的遗传方法来研究生长素信号转导途径。生长素是一种重要的植物生长激素，100多年来一直是人们深入研究的课题。然而，这种基本生长物质的作用机制仍不清楚。拟议的研究几乎肯定会为理解生长素的作用提供重要的见解。这项工作利用了PI构建的一些新的转基因植物系，这些系使用了生长素特异性启动子控制下的报告基因。对转基因植株进行诱变，分离出报告基因表达改变的突变系。一些新的突变体已经被分离出来，提出的研究将导致突变植物的详细特征和最终的基因克隆。由于生长素在植物生长发育中的核心作用，确定参与生长素信号转导的基因对植物生物学研究具有重要意义，并可能最终产生新的作物生产控制方法。拟议的研究还将使PI能够制定一个优秀的长期研究项目，这将证明她作为一名独立研究人员的能力，并使她在寻找永久教师职位时具有竞争力。