ABR: Evolution of 13- and 17- year Periodical Cicadas

ABR：13年和17年周期蝉的演变

• 批准号: 9807113
• 负责人: Chris Simon
• 金额: $ 14.05万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9807113&HistoricalAwards=false
• 关键词: ABR; Evolution; 13; 17; year

9807113 Simon Earlier research explored the evolution of periodical cicadas (Genus Magicicada) using mitochondrial gene sequences, nuclear encoded proteins, and abdominal color. Results from the project included a genetic characterization of spatial patterning of mitochondrial and nuclear genomes, identification of three strong genetic boundaries that do not correspond precisely to the boundaries between periodical cicada species, and a realization of the importance of life history variation in the evolution of this unusual genus. In the process of this work, the evolution of conserved and variable regions of the mitochondrial genome was studied and a number of papers were produced which investigated the value of the secondary structure of the molecule for alignment of nucleo- tides and for understanding patterns of site-to-site variation in the rate of nucleotide evolution. The effects of rate variation on evolutionary tree construction, evaluation of support for groupings of species, and calculation of genetic distances were also explored. In 1998 there will be a joint emergence of the only two broods of 13- and 17-year cicadas whose ranges currently overlap. This will provide a unique opportunity to study the genetic boundaries that we previously discovered. Five genetic techniques for identifying 13- versus 17-year cicadas have been perfected. Life-cycle switching will be examined in greater detail for all six of the periodical cicada species. Previously described genetic boundaries will be explored in an attempt to understand their causes. Finally, a new component will be added to the research, an exploration of the effects of ecological interactions on life-cycle timing and species co-evolution. By studying these unusual cicadas we can learn more about how life-history evolution can dramatically effect species evolution and in so doing answer a broad array of questions about periodi- cal cicadas generated by previous NSF-funded research. The ecological component o f this project will lend an added dimension to the study of co-diversification of this unusual group of insects.

9807113 Simon早期的研究使用线粒体基因序列、核编码蛋白和腹部颜色来探索周期性蝉(Magicicada属)的进化。该项目的结果包括线粒体和核基因组的空间模式的遗传特征，识别出与周期性蝉物种之间的边界不完全对应的三个强大的遗传边界，以及认识到生活史变异在这一特殊属进化中的重要性。在这项工作的过程中，研究了线粒体基因组保守和可变区的进化，并发表了一些论文，调查了分子的二级结构对于核苷酸比对和了解核苷酸进化速度的位置到位置变化的模式的价值。此外，还探讨了速率变异对进化树构建、物种分类支持度的评估和遗传距离计算的影响。1998年将联合出现仅有的两窝13年和17年的蝉，它们的活动范围目前是重叠的。这将为研究我们之前发现的遗传边界提供一个独特的机会。用于识别13年蝉和17年蝉的五种基因技术已经完善。将对所有六种周期性蝉的生命周期转换进行更详细的研究。前面描述的遗传界限将被探索，以试图了解其原因。最后，这项研究将增加一个新的组成部分，探索生态相互作用对生命周期计时和物种共同进化的影响。通过研究这些不寻常的蝉，我们可以更多地了解生活史进化如何戏剧性地影响物种进化，并在这样做的同时回答了一系列关于周期蝉的问题，这些问题是由NSF资助的先前研究产生的。这个项目的生态部分将为研究这一不寻常的昆虫群体的共同多样性提供一个额外的维度。