Exploring Endosymbiont Biodiversity and Complexity in the Family Cicadidae

探索蝉科内共生生物多样性和复杂性

• 批准号: 1655891
• 负责人: Chris Simon
• 金额: $ 84.7万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-15 至 2023-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1655891&HistoricalAwards=false
• 关键词: Exploring; Endosymbiont; Biodiversity; Complexity; Family

All organisms possess beneficial microbial partners (symbionts) that contribute to their health and survival. Many of these live in the gut and are known as the microbiome. Insect symbionts are theorized to bring new features that allow species formation and habitat invasions. In the case of insects that feed on food lacking important nutrients, highly specialized microbes inhabit organs in the abdomen and provide essential amino acids and vitamins. The major goal of this proposal is to study the interactions between insects and microbes and how they function together in a mutually beneficial relationship. The roles of endosymbionts in speciation and biogeography are largely unexplored and their diversity and evolution is poorly known outside a handful of model systems. This research uses cicadas as a study system because they have a particularly deficient diet and are diverse and spread across the globe. DNA data will be used to construct a dated evolutionary tree to study how the cicadas and their symbiont partners have spread around the world and diversified into new species. This information will be useful in general understanding how microorganisms benefit their hosts and specifically in the study of invasions of beneficial and pest insects of agricultural importance. Biodiversity information for cicadas and their symbionts will be disseminated to scientists and the general public by journal publications, society meetings, and the World Wide Web. Citizen science projects and biology lab exercises will be designed and made available. Future scientists will be trained in multiple laboratories, including one postdoc and two graduate students; many undergraduate participants will interact with scientists internationally via the web. Cicada primary endosymbionts have already been demonstrated to include examples of evolution that are surprisingly different from those of other insects, such as rampant symbiont genome duplication/lineage splitting in diverse cicada lineages followed by complementary gene loss and pseudogene formation. To understand the biogeography and evolution of host-symbiont consortia, an existing cicada phylogeny created previously by the research team involved in this work will be expanded and used as a framework. Ecological and symbiont data mapped onto the tree will test hypotheses about the relative timing of changes in cicada habitat association and symbiont consortium membership. This work asks: 1) Does the gain of a secondary (facultative) endosymbiont facilitate the breakdown and or loss of primary (obligate) endosymbiont? Or, 2) Does the breakdown or loss of the obligate endosymbiont allow the invasion of a secondary endosymbiont? Similarly, 3) Do changes in the gut microbiota affect primary endosymbionts (keeping in mind that gut microbiota are known to synthesize essential amino acids for some hosts). Finally, 4) Is symbiont consortium membership related to changes in the external environment (both biotic and abiotic) such as the invasion of new habitats with different environmental conditions and host plants. Improved high-throughput genomic sequencing methods and probes for conserved endosymbiont genes will simultaneously expand the framework Cicadidae evolutionary tree while generating genetic datasets for both of the primary cicada endosymbionts. Surveys of cicada symbiotic partners using metagenomic sequencing (of bacteriomes and fat bodies for endosymbionts) and amplicon sequencing (of gut contents for microbiome studies) will allow the exploration of microbial and fungal symbiont biodiversity. Our hypothesis testing will facilitate exploration of environmental versus evolutionary drivers of biodiversity innovation. New information on the evolution of bacterial endosymbiont genomes will also be produced.

所有生物体都拥有有益的微生物伴侣（共生体），有助于它们的健康和生存。其中许多生活在肠道中，被称为微生物组。理论上，昆虫共生体会带来新的特征，从而允许物种形成和栖息地入侵。在以缺乏重要营养素的食物为食的昆虫的情况下，高度专业化的微生物栖息在腹部器官中并提供必需的氨基酸和维生素。该计划的主要目标是研究昆虫和微生物之间的相互作用，以及它们如何在互利关系中共同发挥作用。内共生体在物种形成和植物地理学中的作用在很大程度上尚未被探索，它们的多样性和进化在少数模型系统之外知之甚少。这项研究使用蝉作为研究系统，因为它们的饮食特别缺乏，而且种类繁多，分布在地球仪各地。DNA数据将用于构建一个过时的进化树，以研究蝉及其共生伙伴如何在世界各地传播并多样化为新物种。这些信息对于全面了解微生物如何使其宿主受益，特别是对于研究具有农业重要性的有益昆虫和害虫的入侵非常有用。蝉及其共生体的生物多样性信息将通过期刊出版物、学会会议和万维网传播给科学家和公众。将设计和提供公民科学项目和生物实验室练习。未来的科学家将在多个实验室接受培训，包括一名博士后和两名研究生;许多本科生参与者将通过网络与国际科学家互动。蝉的初级内共生体已经被证明包括进化的例子，是令人惊讶的不同于其他昆虫，如猖獗的共生体基因组复制/谱系分裂在不同的昆虫谱系随后的互补基因丢失和假基因的形成。为了了解宿主共生体联盟的地理和进化，参与这项工作的研究小组先前创建的现有共生体基因将被扩展并用作框架。映射到树上的生态和共生体数据将测试的假设的相对时间的变化，栖息地协会和共生体财团成员。这项工作问：1）是否获得一个次要的（兼性）内共生体促进破坏和/或损失的主要（专性）内共生体？或者，2）专性内共生体的破坏或丧失是否允许次级内共生体的入侵？同样，3）肠道微生物群的变化是否会影响主要内共生体（请记住，已知肠道微生物群会为某些宿主合成必需氨基酸）。最后，4）共生体联合体成员资格是否与外部环境（生物和非生物）的变化有关，例如具有不同环境条件和宿主植物的新栖息地的入侵。改进的高通量基因组测序方法和保守的内共生体基因的探针将同时扩大蝉科进化树的框架，同时生成两个主要的内共生体的遗传数据集。使用宏基因组测序（内共生体的细菌组和脂肪体）和扩增子测序（微生物组研究的肠道内容物）对微生物共生伙伴进行调查，将有助于探索微生物和真菌共生体的生物多样性。我们的假设检验将有助于探索生物多样性创新的环境与进化驱动因素。还将产生关于细菌内共生体基因组进化的新信息。

项目成果

Out of Africa? A dated molecular phylogeny of the cicada tribe Platypleurini Schmidt (Hemiptera: Cicadidae), with a focus on African genera and the genus Platypleura Amyot & Audinet‐Serville

• DOI: 10.1111/syen.12360
• 发表时间: 2019-10
• 期刊: Systematic Entomology
• 影响因子: 4.8
• 作者: B. Price;D. Marshall;N. Barker;C. Simon;M. Villet

Evolution and Geographic Extent of a Surprising Northern Disjunct Population of 13-Year Cicada Brood XXII (Hemiptera: Cicadidae, Magicicada)

13年蝉二十二代的令人惊讶的北方分离种群的进化和地理范围（半翅目：蝉科，Magicicada）

• DOI: 10.1093/ae/tmx066
• 发表时间: 2017
• 期刊: American Entomologist
• 作者: Kritsky, Gene;Troutman, Roy;Mozgai, Dan;Simon, Chris;Chiswell, Stephen M;Kakishima, Satoshi;Sota, Teiji;Yoshimura, Jin;Cooley, John R
• 通讯作者: Cooley, John R

Documenting Single-Generation Range Shifts of Periodical Cicada Brood VI (Hemiptera: Cicadidae: Magicicada spp.)

• DOI: 10.1093/aesa/saab007
• 发表时间: 2021-04-13
• 期刊: ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA
• 影响因子: 2.3
• 作者: Cooley, John R.;Marshall, David C.;Simon, Chris
• 通讯作者: Simon, Chris

Cicada fossils (Cicadoidea: Tettigarctidae and Cicadidae) with a review of the named fossilised Cicadidae

• DOI: 10.11646/zootaxa.4438.3.2
• 发表时间: 2018-06-22
• 期刊: ZOOTAXA
• 影响因子: 0.9
• 作者: Moulds, M. S.

Psychoactive plant- and mushroom-associated alkaloids from two behavior modifying cicada pathogens

• DOI: 10.1016/j.funeco.2019.06.002
• 发表时间: 2019-10-01
• 期刊: FUNGAL ECOLOGY
• 影响因子: 2.9
• 作者: Boyce, Greg R.;Gluck-Thaler, Emile;Kasson, Matt T.
• 通讯作者: Kasson, Matt T.