RUI: Molecular and Biochemical Physiology of H+ Excretion in the Gills of Marine Fish

RUI：海鱼鳃中 H 排泄的分子和生化生理学

• 批准号: 9808141
• 负责人: James Claiborne
• 金额: $ 24.3万
• 依托单位: Georgia Southern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9808141&HistoricalAwards=false
• 关键词: RUI; Molecular; Biochemical; Physiology; H+

9808141 Claiborne The fish gill is a multi-purpose organ. Analogous to the lungs in land-dwelling animals, the gills allow the fish to breathe oxygen and excrete carbon-dioxide. Further, the gills perform functions of the kidneys as they eliminate toxic metabolic acid from the animal. Dr. Claiborne has hypothesized that saltwater fish may excrete acid using a family of sodium hydrogen exchanger proteins (NHE) within the gills. These proteins were first discovered in mammals but our preliminary data indicate that they are also present in more primitive animals like fish. The PI will investigate the genetic regulation and protein expression of the NHEs in the gills of two different marine species - the sculpin and the killifish. This should provide new insight into the physiology of gill acid-base and ion regulation and shed new light on evolutionary relationships between the vertebrates. Further, since it is known that the cellular mechanisms within the gills are similar to those currently being studied in the mammalian kidney, a new understanding of the physiology of the kidney and other organs may also be achieved.

9808141 Claiborne 鱼鳃是一种多用途器官。 类似于陆地动物的肺部，鳃让鱼呼吸氧气并排出二氧化碳。 此外，鳃还发挥肾脏的功能，消除动物体内有毒的代谢酸。 克莱伯恩博士推测，咸水鱼可能利用鳃内的钠氢交换蛋白 (NHE) 家族来排泄酸。 这些蛋白质首先在哺乳动物中发现，但我们的初步数据表明它们也存在于更原始的动物（如鱼类）中。 PI 将研究两种不同海洋物种（杜父父鱼和鳉鱼）鳃中 NHE 的遗传调控和蛋白质表达。 这将为鳃酸碱和离子调节的生理学提供新的见解，并为脊椎动物之间的进化关系提供新的线索。 此外，由于已知鳃内的细胞机制与目前在哺乳动物肾脏中研究的细胞机制相似，因此也可能对肾脏和其他器官的生理学产生新的理解。