Characterization of Ubiquitin Pathway in Higher Plants

高等植物中泛素途径的表征

• 批准号: 9808791
• 负责人: Judy Callis
• 金额: $ 44万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9808791&HistoricalAwards=false
• 关键词: Characterization; Ubiquitin; Pathway; Higher; Plants

9808791 Callis This proposal will investigate the regulation of protein degradation in higher plants and examine the functional roles that the highly conserved proteolytic ubiquitin pathway serves in controlling plant growth processes. The proposal will focus on the AUX/IAA family of proteins that are highly expressed in response to the plant hormone, auxin but rapidly degrade. Major objectives of this proposal are to determine which amino acids constitute the degradation signal, to identify the proteolytic mechanisms involved in AUX/IAA degradation, and to isolate mutants defective in the degradation of the AUX/IAA proteins. Another major objective of the proposal is to investigate the expression and function of RUB, a protein related to ubiquitin that is involved in auxin responses in plants. Together, the proposed studies will elucidate how controlled proteolysis functions. in auxin and other signaling processes. In addition, the work will increase our fundamental understanding of ubiquitin pathway function in eucaryotic organism.

9808791 Callis本研究将探讨高等植物蛋白质降解的调控，并研究高度保守的蛋白水解泛素途径在控制植物生长过程中的功能作用。 该提案将集中在AUX/IAA家族的蛋白质，这些蛋白质在响应植物激素生长素时高度表达，但迅速降解。 该建议的主要目的是确定哪些氨基酸构成降解信号，以确定涉及AUX/IAA降解的蛋白水解机制，并分离AUX/IAA蛋白降解缺陷的突变体。 该提案的另一个主要目标是研究RUB的表达和功能，RUB是一种与植物生长素反应相关的泛素蛋白。 总之，拟议的研究将阐明如何控制蛋白水解功能。在生长素和其他信号传递过程中。 此外，本研究也为深入了解真核生物中泛素通路的功能奠定了基础。