Neuronal cell recognition in Drosophila

果蝇的神经细胞识别

• 批准号: 127303345
• 负责人: Professor Dr. Thomas Hummel
• 金额: --
• 依托单位: Department für Neurowissenschaften und Entwicklungsbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/127303345?language=en
• 关键词: Neuronal; cell; recognition; Drosophila

The segregation of axon and dendrite projections into distinct synaptic units is a fundamental principle of nervous system organization and the structural basis for information processing in the brain. The molecular mechanisms that allow projecting neurons to recognize their synaptic partners and initiate synaptogenesis are largely unknown. Using a systematic genetic mosaic approach in the Drosophila visual and olfactory system, we have identified distinct control mechanisms, which are critical for sensory axons to connect to their correct CNS partner neurons. The research proposed in this application is aimed at understanding the molecular basis of cellular recognition specificity that coordinates the assembly of multiple neuronal subtypes into functionally distinct microcircuits. To this end we intend to explore, in a comparative approach between the visual and the olfactory system, the molecular basis of two different developmental mechanisms for the generation of neuronal recognition identity. First, by determining how the temporal expression dynamics of the Zn finger protein Sequoia in projecting photoreceptor neurons controls layer specific innervation. And second, by characterizing how the chromatin regulator Psc coordinates the differentiation of sensory and synaptic identity of olfactory neurons. From these analyses we are expecting to obtain a better understanding of how the specificity of neural brain networks is encoded in the genome.

轴突和树突投影分为不同的突触单元是神经系统组织的基本原理，也是大脑信息处理的结构基础。允许投射神经元识别其突触伴侣并启动突触发生的分子机制在很大程度上是未知的。使用果蝇视觉和嗅觉系统中的系统遗传镶嵌方法，我们确定了不同的控制机制，这对于感觉轴突连接到其正确的CNS伴侣神经元至关重要。本应用中提出的研究旨在了解细胞识别特异性的分子基础，该特异性将多个神经元亚型组装成功能上不同的微电路。为此，我们打算在视觉和嗅觉系统之间的比较方法中探索，这是两种不同发育机制的分子基础，用于产生神经元识别身份。首先，通过确定Zn指蛋白红杉在投射光感受器神经元中的时间表达动力学如何控制层特定的神经。其次，通过表征染色质调节剂PSC如何协调嗅觉神经元的感觉和突触认同的分化。从这些分析中，我们期望可以更好地了解神经脑网络的特异性如何在基因组中编码。