Loss of Antibody Productivity by Hybridomas: Is DNA Loss Responsible?

杂交瘤导致抗体生产力丧失：DNA 丢失是罪魁祸首吗？

• 批准号: 9809820
• 负责人: Carole Heath
• 金额: $ 7.98万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9809820&HistoricalAwards=false
• 关键词: Loss; Antibody; Productivity; Hybridomas; DNA

9809820 Heath The instability of fused cell lines such as hybridoma remains a major complication in the production of commercially important monoclonal antibodies. In previous work, these PIs demonstrated that the diminished productivity of hybridoma cells is due to the formation of a non-productive cell subpopulation rather than a weakening across the line. The non-productive cells were not producing the essential antibody heavy chain (HC). The loss of HC production can be the result of DNA loss or mutation or some kind of event affecting mRNA production, regulation, or translation. In this proposal the DNA-loss hypothesis will be investigated. ***

融合细胞系如杂交瘤的不稳定性仍然是生产商业上重要的单克隆抗体的主要复杂因素。 在之前的工作中，这些PI证明杂交瘤细胞生产力的下降是由于非生产性细胞亚群的形成，而不是整个生产线的减弱。 非生产性细胞不产生必需抗体重链（HC）。 HC产生的损失可能是DNA丢失或突变或某种影响mRNA产生、调节或翻译的事件的结果。 在这个提议中，DNA丢失假说将被调查。 ***