Engineering Research Equipment Grant: HPLC

工程研究设备补助金：HPLC

• 批准号: 9105215
• 负责人: Carole Heath
• 金额: $ 1.79万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105215&HistoricalAwards=false
• 关键词: Engineering; Research; Equipment; Grant; HPLC

The primary purpose for this High Performance Liquid Chromatography (HPLC) system is an investigation of the stability of monoclonal antibody (MAb) production by hybridomas and quadromas, the fusion product of two hybridomas, during long-term continuous culture in suspension and immobilized systems. Using an optimized HPLC process, the number and concentrations of antibodies for both parent hybridomas, and the quadroma, will be monitored during long-term continuous culture. Results will be integrated with concurrent flow cytometry and metabolic studies in an effort to elucidate mechanisms for improving the stability of MAb production by hybridomas and quadromas.

该高效液相色谱(HPLC)系统的主要目的是考察单抗生产的稳定性。杂交瘤和四色瘤，两株杂交瘤的融合产物，在悬浮和固定化系统中长期连续培养。使用优化的高效液相程序，亲本杂交瘤和四倍体抗体的数量和浓度，将在长期连续培养过程中进行监测。结果将与同时进行的流式细胞术和代谢研究相结合，努力阐明通过以下方式提高单抗生产稳定性的机制杂交瘤和四色瘤。