The Generation of a Tomato EST Database

番茄EST数据库的建立番茄植物长期以来一直是植物生物学研究各个方面的热门物种，包括遗传学，发育，生理学和病理学。今天，许多关于番茄的研究集中在果实发育和病原体反应上，因为在这些领域，番茄仍然是理想的模型。近年来，基因组学已经上升到现代生物学研究的前沿，现在为植物生物学提供了巨大的机会。从生物体中产生表达序列标签（EST）是最广泛使用的基因组学方法之一。这些是从随机选择的cDNA克隆获得的短序列，并且是鉴定组织中表达的大量基因的非常经济有效的方式。一旦生成，这些序列是大量实验方法的宝贵资源，包括作图，表达研究和基因组注释。该项目将从各种番茄组织中产生90，000个EST，包括大多数植物中存在的核心组织（芽，根，发育中的花药和胚珠以及发育中的种子），以及番茄研究人员特别感兴趣的组织，如成熟果实和患病组织。这90，000个序列将用于构建番茄EST数据库，其中来自相同基因的EST被分组在一起并被视为单个实体。然后对这些基因进行注释，以确定每个基因的可能功能。关于每个EST来源的信息也将成为该数据库的一部分，因此将有可能识别组织特异性基因，以及响应攻击病原体而启动的基因。预计该项目的序列和克隆将成为番茄研究人员使用的工具集合的一部分，实际上研究任何其他物种的研究人员。来源：1。15万个番茄EST序列（GenBank）2.汇编和注释的番茄EST/cDNA的公众可访问数据库，每季度更新一次（TIGR番茄基因索引; http：//www.tigr.org/tdb/tgi/lgi/）。

The Generation of a Tomato EST Database. The tomato plant has long been a popular species for various aspects of plant biology research including genetics, development, physiology and pathology. Today much of the research on tomato concentrates on fruit development and pathogen response since in these areas tomato still stands out as an ideal model. In recent years, genomics has risen to the forefront of modern biological research and now provides great opportunities for plant biology. One of the most widely used genomics approaches is to generate Expressed Sequence Tags (ESTs) from an organism. These are short sequences obtained from cDNA clones selected at random and are a very cost effective way of identifying a large number of genes expressed in a tissue. Once generated these sequences are a valuable resource for a large number of experimental approaches including mapping, expression studies, and genome annotation. This project will generate 90,000 ESTs from a variety of tomato tissues including core tissues present in most plants (shoots, roots, developing anthers and ovules, and developing seeds), as well as tissues of particular interest to tomato researchers such as ripening fruits, and diseased tissues. These 90,000 sequences will be used to construct a tomato EST database in which the ESTs from the same gene are grouped together and treated as a single entity. These will then be annotated to identify possible functions of each gene. Information about the source of each EST will also be part of this database, and therefore it will be possible to identify tissue-specific genes, and genes switched on in response to attacking pathogens. It is expected that the sequences and the clones from this project will become part of the collection of tools used by tomato researchers, and indeed researchers working on any number of other species. Deliverables (source): 1. 150,000 tomato EST sequences (GenBank)2. Publicly-accessible database of assembled and annotated tomatoESTs/cDNAs, updated quarterly (TIGR tomato Gene Index;http://www.tigr.org/tdb/tgi/lgi/).