CO2 Effects on CHO Cell Growth, Metabolism, and Glycosylation
CO2 Effects on CHO Cell Growth, Metabolism, and Glycosylation
批准号:
9813479
负责人:
William Miller
金额:
$35.99万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-01-01 至 2002-12-31
中文摘要
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英文摘要
This proposal deals with the effects of carbon dioxide buildup in the production of proteins by mammalian cells. CO2, an oxidation by-product, is known to inhibit cell growth and probably to alter important glycosylation patterns. The impact of CO2 on culture parameters such as rates, clycosylation, apoptosis, etc., and its interaction with other process variables such as pH, osmolality, and ammonia concentration, will be determined. Two CHO cell lines will be used and the impact on three proteins, tPA, IFN-gamma, and a neural cell adhesion molecule (NCAM) will be studied. A mechanism for growth inhibition by CO2 will be sought.
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