Trace Metal Limitation of Phytoplankton Productivity: Combined Immunological, Geochemical, and Growth Assay Approaches in Lake Superior

虽然最近的一些研究已经证实，各种海洋环境中的初级生产可能受到痕量金属可用性的限制，但对淡水系统，甚至北美五大湖的内海系统还没有类似的研究。在本项目中，来自明尼苏达大学、罗格斯大学和州立鲍灵格林大学的研究人员将研究苏必利尔湖微量金属限制初级生产的存在、机制、时空程度和意义。他们将采取三管齐下的办法。首先，为了量化和表征总和生物活性微量金属浓度，将测定溶液、悬浮颗粒和野外浮游生物中的Al、Fe、Mn、Zn、Cu、Cd和Co。其次，利用免疫学和荧光法对田间藻类金属缺乏症进行评价。第三，采用痕量金属富集实验，在实验室进行局限性实验评估。这三个地点将被选择利用现有的数据，这些数据来自nsf赞助的风筝项目。

ABSTRACTOCE-9819324 / OCE-9902660 / OCE- 9902658Although a number of recent studies have verified that primary production in various marine environments may be limited by trace metal availability, there has not yet been a similar body of research for freshwater systems, even the inland sea system of the North American Great Lakes. In this project researchers from the University of Minnesota, Rutgers University, and Bowling Green State University will investigate the existence, mechanisms, spatial-temporal extent, and significance of trace metal limitation to primary production in Lake Superior. They will take a three-pronged approach. First, to quantify and characterize total and bioactive trace metal concentrations, Al, Fe, Mn, Zn, Cu, Cd, and Co would be determined in solution, in suspended particles, and in plankton in the field. Secondly, immunological and fluorescence assays would be used to assess metal deficiency in algae in the field. Third, trace metal enrichment experiments would be used to assess limitation experimentally in the laboratory. The three field sites would be chosen to take advantage of existing data available from the NSF-sponsored KITES program.