Collaborative Research: Acquisition of nitrogenase metal cofactors in soils: role of metallophores and limitation of N2-fixation

合作研究:土壤中固氮酶金属辅助因子的获取:金属团的作用和固氮的限制

基本信息

  • 批准号:
    1024545
  • 负责人:
  • 金额:
    $ 3.4万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-09-01 至 2014-08-31
  • 项目状态:
    已结题

项目摘要

Intellectual merit: N2 fixation is effected exclusively by bacteria and archea using the enzyme nitrogenase, which contains Fe and Mo as metal cofactors in its most active form, and Fe and V, or Fe-only in its alternative forms. The aim of this project is to elucidate how the mechanisms and kinetics of bacterial acquisition of Fe, Mo and V from soils may limit or control N2 fixation rates. N2-fixing bacteria produce siderophores (or ?iron carriers?) to bind iron in the external medium and take up the resulting Fe-siderophore complexes. Our recent work has shown that the siderophores are actually ?metallophores? used in the uptake of Mo and V, along with Fe. The biological acquisition of the various nitrogenase metal cofactors thus depends on their binding by bacterial metallophores. This project is organized around two major hypotheses: 1. Free-living N2-fixing bacteria excrete metallophores that are particularly efficient at capturing the metal (Mo, V or Fe) that is limiting N2 fixation; 2. Because of both competition with other metals for metallophore binding and scarcity in soils, Mo is inherently difficult to acquire and free-living N2-fixing bacteria often use alternative nitrogenases, particularly the V-nitrogenase, to fix N2. These hypotheses will be tested through a combination of laboratory and field experiments. Field studies will focus on sites where Mo may be limiting and employ molecular biological techniques to identify alternative nitrogenases.Broader Impacts: One postdoctoral researcher, one graduate student and several undergraduates will work on this project and be mentored by the PIs. Students from the local junior colleges will participate in the summer field research. The PIs will participate in the Quest summer program for school teachers.
智力优势:固氮完全由细菌和古细菌利用酶氮酶来完成,其中最活跃的形式含有铁和钼作为金属辅因子,而铁和V,或仅含铁的替代形式。本项目的目的是阐明细菌从土壤中获取铁、钼和钒的机制和动力学如何限制或控制氮的固定速率。固氮细菌产生铁载体(或?铁载体?)结合外部介质中的铁,并吸收产生的铁铁载体配合物。我们最近的研究表明,铁载体实际上是金属载体。用于吸收Mo和V以及Fe。因此,各种氮酶金属辅因子的生物获取取决于它们与细菌金属载体的结合。这个项目是围绕两个主要假设来组织的:1。自由生活的固氮细菌分泌的金属载体在捕获限制固氮的金属(Mo, V或Fe)方面特别有效;2. 由于与其他金属结合的竞争以及土壤中的稀缺性,Mo天生难以获得,自由生活的固氮细菌通常使用替代的固氮酶,特别是v -固氮酶来固定N2。这些假设将通过实验室和实地实验相结合的方式进行检验。实地研究将集中在Mo可能限制的地点,并采用分子生物学技术来确定替代的氮酶。更广泛的影响:一名博士后研究员,一名研究生和几名本科生将参与该项目,并由pi指导。来自当地大专院校的学生将参加暑期实地调研。这些pi将参加面向学校教师的Quest暑期项目。

项目成果

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Anthony Aufdenkampe其他文献

Elemental and mineralogical changes in soils due to bioturbation along an earthworm invasion chronosequence in Northern Minnesota
  • DOI:
    10.1016/j.apgeochem.2011.03.047
  • 发表时间:
    2011-06-01
  • 期刊:
  • 影响因子:
  • 作者:
    Kathryn Resner;Kyungsoo Yoo;Cindy Hale;Anthony Aufdenkampe;Alex Blum;Stephen Sebestyen
  • 通讯作者:
    Stephen Sebestyen
Phytoplankton abundance and contributions to suspended particulate matter in the Ohio, Upper Mississippi and Missouri Rivers
  • DOI:
    10.1007/s00027-011-0190-y
  • 发表时间:
    2011-03-05
  • 期刊:
  • 影响因子:
    1.800
  • 作者:
    Paul A. Bukaveckas;Amy MacDonald;Anthony Aufdenkampe;John H. Chick;John E. Havel;Richard Schultz;Ted R. Angradi;David W. Bolgrien;Terri M. Jicha;Debra Taylor
  • 通讯作者:
    Debra Taylor
Carbon–mineral interactions along an earthworm invasion gradient at a Sugar Maple Forest in Northern Minnesota
  • DOI:
    10.1016/j.apgeochem.2011.03.037
  • 发表时间:
    2011-06-01
  • 期刊:
  • 影响因子:
  • 作者:
    Amy Lyttle;Kyungsoo Yoo;Cindy Hale;Anthony Aufdenkampe;Stephen Sebestyen
  • 通讯作者:
    Stephen Sebestyen

Anthony Aufdenkampe的其他文献

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{{ truncateString('Anthony Aufdenkampe', 18)}}的其他基金

REU/RET Site: Collaborative Research: Introducing Critical Zone Observatory Science to Students and Teachers
REU/RET 网站:合作研究:向学生和教师介绍关键区域天文台科学
  • 批准号:
    1263222
  • 财政年份:
    2013
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Continuing Grant
Collaborative Research: SI2-SSI: The Community-Driven BiG CZ Software System for Integration and Analysis of Bio- and Geoscience Data in the Critical Zone
合作研究:SI2-SSI:社区驱动的 BiG CZ 软件系统,用于整合和分析关键区域的生物和地球科学数据
  • 批准号:
    1339834
  • 财政年份:
    2013
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Standard Grant
Integrated Data Management System for Critical Zone Observatories
关键区域观测站综合数据管理系统
  • 批准号:
    1153164
  • 财政年份:
    2012
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Standard Grant
Integrated Data Management System for Critical Zone Observatories
关键区域观测站综合数据管理系统
  • 批准号:
    1332257
  • 财政年份:
    2012
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Standard Grant
EarthCube Domain End-User Workshop: Engaging the Critical Zone community to bridge long tail science with big data
EarthCube 域最终用户研讨会:让关键区域社区参与到长尾科学与大数据之间的桥梁
  • 批准号:
    1252238
  • 财政年份:
    2012
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Standard Grant
MRI: Acquisition of a High-Sensitivity Light Stable Isotope Ratio Mass Spectrometer for Critical Zone Studies
MRI:购买高灵敏度光稳定同位素比质谱仪用于关键区域研究
  • 批准号:
    1126627
  • 财政年份:
    2011
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Standard Grant
Collaborative Research: Geomorphodynamic Modulation of Biogeochemical Fluxes and Basin Stratigraphy of the Fly River
合作研究:生物地球化学通量的地貌动力学调节和弗莱河盆地地层学
  • 批准号:
    0742478
  • 财政年份:
    2008
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Standard Grant
Collaborative Research: Episodic, ENSO-Orchestrated Carbon Sequestration in Amazonian River Basins by Erosion-Sedimentation Processes
合作研究:亚马逊河流域通过侵蚀-沉积过程间歇性、ENSO 精心安排的碳封存
  • 批准号:
    0404169
  • 财政年份:
    2004
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Standard Grant
International Research Fellowship Program: River Metabolism Within the Amazon Basin: Constraints on the ROMBUS Model
国际研究奖学金计划:亚马逊流域内的河流代谢:ROMBUS 模型的约束
  • 批准号:
    0107270
  • 财政年份:
    2002
  • 资助金额:
    $ 3.4万
  • 项目类别:
    Fellowship Award

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合作研究:LTREB:资源可用性、获取和动员对于可变环境中生命史权衡演变的重要性。
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