A census of viruses through the drinking water cycle

沃特博姆病毒具有很高的公共卫生意义，但到目前为止被低估了。在水监测和监督法规中，到目前为止，病毒检测并不是强制性的。这一点反映在现有的方法学汇辑中。到目前为止，检测不同类型水域(废水、地表水、地下水、饮用水)中各种类型病毒的方法不够灵敏。一些最重要的水媒病毒，如诺如病毒，只能通过聚合酶链式反应方法检测。在水媒病毒暴发的情况下，在没有可靠的检测方法的情况下，往往无法查明潜在的情况和原因。这同样适用于生物犯罪或恐怖主义行为。因此，最重要的是进一步开发在不同类型的水域中进行敏感和可靠的病毒检测的方法，这些方法在技术上容易在短时间内完成，在小容量中提供足够的大范围病毒浓度，具有高的病毒回收率，不会太昂贵，并将产生可重复的结果。在这项提议中，将与个别项目合作伙伴合作，开发浓缩大量水的方法，以便在水样中同时检测到大量病毒。在实验室成功开发和测试后，将对这些方法在不同水和水处理步骤中的使用进行评估，以进行定量和定性的病毒分析。

Waterbome viruses have a high but so far underestimated public health significance. In water monitoring and surveillance regulations, virus detection is until now not mandatory. This is reflected in the methodological repertoire available. To date, methods for detecting the various types of viruses in different types of waters (waste water, surface water, groundwater, drinking water) are insufficiently sensitive. Some of the most important waterborne viruses like noroviruses can only be detected by PCR methods. In the case of waterborne virus outbreaks, underlying circumstances and causes frequently cannot be clarified in the absence of reliable detection methodology. The same would apply to acts of biological crime or terrorism. It is thus of utmost importance to further develop methods for sensitive and reliable virus detection in different types of waters which are technically easy to accomplish in a short time, provide a sufficient concentration of a large range of viruses in a mall volume, have a high virus recovery rate, will not be too costly, and will deliver reproducible results. In this proposal methods for concentrating large volumes of water by which a large spectrum of viruses can be simultaneously detected in water samples will be developed in cooperation with individual project partners. After successful development and testing in the lab, the methods will be evaluated for its use in different waters and water treatment steps for quantitative and qualitative virus analysis.