SBIR Phase I: Magnetic Polystyrene Microspheres for Bio-magnetic Separation

9860290 这个小企业创新研究第一阶段项目将开发一种新技术，制造均匀的聚苯乙烯涂层铁颗粒，用于生物磁分离。生物磁分离是一种新兴的技术，利用磁性，有时与传统的分离或鉴定方法，以纯化细胞，蛋白质和核酸。由于其可扩展性，效率，简单性，温和的条件，易于自动化和低成本，它们越来越有吸引力。然而，首先制备聚合物，然后尝试将超细磁铁矿（例如γ-Fe 3 〇 4）或铁氧体（例如γ-Fe 2 〇 3）吞没到聚合物胶体颗粒中的当前方法不是有效的或有效的。结果，最终产物具有低的磁铁矿或铁氧体含量，因此生物磁性分离的效率低。与此同时，供应商很少，一些产品质量较差，在珠子尺寸和珠子表面化学方面几乎没有选择性。在这第一阶段的努力，MMI提出了合成纳米级铁苯乙烯胶体溶液的溶剂化金属原子分散（SMAD）的方法，然后通过聚合苯乙烯。聚苯乙烯包覆的铁粒子经过适当的表面修饰后有望成为超顺磁性的，并在生物磁分离中具有上级性能。 聚苯乙烯包被的铁颗粒有许多应用，例如细胞分离、免疫测定、乳胶凝集、细胞标签、血流标记、色谱测定、血细胞刺激、磁共振成像造影剂、细胞培养中的微载体、DNA探针/PCR。

9860290 This Small Business Innovation Research Phase I project will develop a new technology to make uniform polystyrene coated iron particles for bio-magnetic separations. Bio-magnetic separations are an emerging technology using magnetism, sometimes with conventional separations or identification methods, to purify cells, proteins and nucleic acids. They are increasingly appealing due to their scalability, efficiency, simplicity, mild conditions, ease of automation, and low cost. However, current methods that make polymers first followed by attempts to engulf ultra-fine magnetite (e.g. y-Fe304) or ferrite (e.g. y-Fe203) into the polymeric colloidal particles are not efficient, or effective. As a result, the final product has low magnetite or ferrite content hence low efficiency in bio-magnetic separation. At the same time, there are few suppliers, some products have poor quality, and there is little selectivity in terms of size of the beads and surface chemistry of the beads. In this Phase I effort, MMI proposes to synthesize nanoscale Fe styrene colloidal solutions by the solvated metal atoms dispersion (SMAD) method, followed by polymerizing styrene. The polystyrene-coated iron particles are expected to be superparamagnetic and have superior properties in bio-magnetic separations after proper surface modification. There are many applications for the polystyrene coated iron particles, such as cell separation, immunoassays, latex agglutination, cell tags, blood flow markers, chromatographic assays, blood cell stimulation, magnetic resonance imaging contrast agents, micro-carriers in cell culture, DNA probes/PCR.