411POWRE: Analysis of Microbial Communities by Coupled Polymerase Chain Reaction and Dematuring Gradient Gel Electrophoresis as an Indicator of in Situ Bioremediatio

411POWRE：通过耦合聚合酶链反应和变性梯度凝胶电泳分析微生物群落作为原位生物修复的指标

• 批准号: 9870561
• 负责人: Junko Munakata Marr
• 金额: $ 8.5万
• 依托单位: Colorado School of Mines
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-15 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9870561&HistoricalAwards=false
• 关键词: 411POWRE; Analysis; Microbial; Communities; Coupled

9870561 Marr The objective of this research is to establish a foundation for the verification of in situ bioremediation. The proposed research will test the hypothesis that the molecular biology tools of polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) can be used to monitor the succession of microbial populations associated with in situ biodegradation of chlorinated solvents. PCR and DGGE have been successfully applied to natural microbial communities to elucidate microbial diversity and identify the specific populations present in uncontaminated aquatic, sediment and soil environments. Specific objectives are to 1) optimize the application of PCR and DGGE to distinguish microbial species in uncontaminated methanotrophic soil, 2) simultaneously monitor trichloroethylene degradation and species diversity as measured by DGGE resolution of PCR-amplified 16S rDNA and 3) identify the predominant species over time via type-specific phylogenetic and catabolic gene probes and by 16S rDNA sequence analysis. The ultimate impact of this research will be to enhance our knowledge of bioremediation and to provide tools to monitor and optimize operating conditions during in situ bioremediation of contaminated groundwater sites. This project will allow the PI to take a leadership position among researchers in the area of disinfection processes by focusing her research in an area were there are significant knowledge gaps

9870561马尔本研究的目的是为原位生物修复的验证奠定基础。拟议的研究将测试的假设，聚合酶链反应（PCR）和变性梯度凝胶电泳（DGGE）的分子生物学工具，可用于监测与氯化溶剂的原位生物降解相关的微生物种群的演替。PCR和DGGE已成功应用于自然微生物群落，以阐明微生物多样性，并确定存在于未受污染的水，沉积物和土壤环境中的特定种群。具体目标是：1）优化PCR和DGGE的应用，以区分未污染的甲烷营养型土壤中的微生物物种，2）同时监测三氯乙烯降解和物种多样性，通过PCR扩增的16S rDNA的DGGE分辨率和3）确定随时间推移的优势物种，通过类型特异性系统发育和分解代谢基因探针和16S rDNA序列分析。这项研究的最终影响将是提高我们的生物修复知识，并提供工具，监测和优化操作条件，在原位生物修复受污染的地下水网站。该项目将使PI在消毒过程领域的研究人员中占据领导地位，将其研究集中在存在重大知识差距的领域