Acquisition of Scanning Electron Microscope for Multi-User Electron Microscopy Core Facility

为多用户电子显微镜核心设施购置扫描电子显微镜

• 批准号: 9871184
• 负责人: Heide Schatten
• 金额: $ 27.34万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-15 至 2001-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9871184&HistoricalAwards=false
• 关键词: Acquisition; Scanning; Electron; Microscope; Multi

The purpose of this proposal is to request funds for the purchase of a JEOL JSM-6340F Field Emission Scanning Electron Microscope with key optional capabilities including a field emission (FE) electron gun for increased resolution of sample analysis, and a backscattered electron detector for low voltage imaging and analysis of gold-labeled (3, 5, and 10 nm) surface antigens. This new JEOL JSM 6340F FESEM will serve 7 major and 13 minor users from seven different departments in interdisciplinary research and education efforts at the University of Missouri-Columbia (MU). The requested microscope will be housed in the Electron Microscopy Core Facility (EMCF) which is a recently reorganized centralized multi-user core facility that has as its mission to support research and education within the University community and in the region. It was established in 1995 as a result of combining the resources from the College of Agriculture, Food and Natural Resources, College of Arts and Science, College of Veterinary Medicine, and School of Medicine. The need for more modern capabilities has made it essential to ask for funds to purchase a new versatile, reliable, and user-friendly instrument, as the 20-year old JEOL-JSM35 scanning electron microscope cannot satisfy the demands of the present users. Additionally, because the EMCF serves a diversity of research projects and has as it's mission the training and education of future scientists, modern instrumentation is critically important. The new capabilities requested for this multi-user Core Facility will allow (1) high resolution analysis of minimally coated samples of biological plant and animal materials, and of molecular isolates and spreads; (2) high resolution detection of small gold labels down to 3 and 5nm gold-labeling for immuno-electron microscopy; (3) image acquisition and archiving for post-examination analyses; (4) use of the PC-based, fully digital microscope for training computer-age microscopists and resea rchers; (5) the ability to add a cryo-system; and (6) the ability to add enhanced x-ray microanalysis of minimally coated specimens from an EDS system that is planned for future purchase. These features have become essential for major parts of funded projects in agronomy, entomology, plant pathology, cell and molecular biology, veterinary parasitology, plant and animal reproduction, microbiology, and other basic sciences. The requested microscope will also serve to train users and students in a graduate-level Cell and Molecular Electron Microscopy course offered annually. The new microscope will be supervised by the EMCF director, who is assisted by two permanent staff EM technicians. The EMCF is one of six Core Facilities administered by MU's Molecular Biology Program, a state-funded program, that is committed to provide 50% of the capital costs, long-term salaries for the director and staff of the EMCF, and substantial subsidies for existing equipment as well as laboratory renovations and service contracts for all Core instruments.

这项提案的目的是申请资金，用于购买JEOL JSM-6340F场发射扫描电子显微镜，其主要可选功能包括用于提高样品分析分辨率的场发射(FE)电子枪，以及用于金标记(3、5和10 nm)表面抗原的低电压成像和分析的背散射电子探测器。这一新的JEOL JSM 6340F FESEM将为密苏里大学哥伦比亚大学(MU)七个不同系的7个主要用户和13个次要用户提供跨学科研究和教育努力。所要求的显微镜将存放在电子显微镜核心设施(EMCF)，这是一个最近重组的集中式多用户核心设施，其使命是支持大学社区和该地区的研究和教育。它成立于1995年，综合了农业、食品和自然资源学院、文理学院、兽医学院和医学院的资源。由于需要更现代化的功能，因此有必要申请资金购买一种新的多功能、可靠和用户友好的仪器，因为已有20年历史的JEOL-JSM35扫描电子显微镜不能满足目前用户的需求。此外，由于EMCF服务于各种研究项目，并将培训和教育未来的科学家作为其使命，现代仪器设备至关重要。这一多用户核心设施所需的新能力将使：(1)对生物动植物材料的最小涂层样品以及分子隔离和扩散进行高分辨率分析；(2)高分辨率检测小金标，小至3纳米和5纳米金标，用于免疫电子显微镜；(3)图像采集和存档，用于检查后分析；(4)使用个人计算机的全数字显微镜，培训计算机时代的显微镜工作者和研究人员；(5)增加低温系统的能力；以及(6)对计划在未来购买的EDS系统中最小涂层的样品添加增强的X射线显微分析的能力。这些特征已成为农学、昆虫学、植物病理学、细胞和分子生物学、兽医寄生虫学、动植物繁殖、微生物学和其他基础科学等资助项目的重要组成部分。申请的显微镜还将用于对用户和学生进行研究生水平的细胞和分子电子显微镜课程的培训。新的显微镜将由EMCF主任监督，该主任由两名长期工作人员EM技术人员协助。EMCF是墨尔本大学分子生物学计划管理的六个核心设施之一，该计划是一个国家资助的计划，承诺提供50%的资本成本、EMCF主任和工作人员的长期工资、现有设备的大量补贴以及所有核心仪器的实验室翻新和服务合同。