Acquisition of Instrumentation for Peptide Synthesis and Purification
购置肽合成和纯化仪器
基本信息
- 批准号:9871427
- 负责人:
- 金额:$ 5.96万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:1998
- 资助国家:美国
- 起止时间:1998-09-01 至 1999-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The PI proposes the acquisition of instrumentation for the synthesis and purification of short peptides. This instrumentation will help advance the research program of the PI involving spectroscopic characterization of model alpha helices. The infrared (IR) spectra of amide modes of peptides have long been used to estimate secondary structure content and describe conformational dynamics. However, the transition dipole coupling which makes these modes sensitive to backbone structure also causes these modes to be global markers about protein structure and limits their application as specific, local probes. In this study, isotopic labels introduced into the sequence of model helical peptides will be used to disrupt transition dipole coupling and to provide local reporters of peptide structure and environment. These labels will be used to investigate the effect of helix capping and helix dipoles on amide I frequency. In addition, the labeled residues will be used to investigate folding/unfolding processes of model helices focusing on the conformation of a particular residue. The basic information about the effect of local interactions on amide I frequencies will be the basis for developing methodology to use IR spectroscopy as a tool for probing of dissecting specific structural or dynamic properties of proteins. The peptide synthesis instrumentation will make the pursuit of this research project more time and cost efficient by making possible the preparation of isotopically labeled peptides on site. Mount Holyoke College, the nation's oldest continuing liberal arts college for women, has a long history of educating women for careers in scientific research. A key component of this tradition is the collaboration between undergraduate students and faculty on research projects. In order to best prepare students for future research careers, undergraduate research programs must give students as much direct, hands-on experience with the techniques and instrumentation as possible. This instrumentation will enhance the research experience of student collaborators with the PI by exposing them to important, modern techniques of biochemistry.
PI建议收购用于短肽合成和纯化的仪器。该仪器将有助于推进PI的研究计划,包括模型α螺旋的光谱表征。肽的酰胺模式的红外(IR)光谱长期以来被用于估计二级结构含量和描述构象动力学。然而,跃迁偶极耦合,这使得这些模式的骨干结构敏感,也导致这些模式是关于蛋白质结构的全局标记,并限制了它们作为特定的,局部探针的应用。在这项研究中,同位素标记引入到模型螺旋肽的序列将被用来破坏过渡偶极耦合,并提供肽结构和环境的本地报告。这些标签将用于研究螺旋帽和螺旋偶极子对酰胺I频率的影响。此外,标记的残基将用于研究折叠/展开过程的模型螺旋集中在一个特定的残基的构象。本地相互作用对酰胺I频率的影响的基本信息将是开发方法的基础,使用红外光谱作为工具,用于探测解剖蛋白质的特定结构或动力学性质。肽合成仪器将使追求这个研究项目的时间和成本效益更高,使同位素标记的肽的现场制备成为可能。 蒙特霍利奥克学院是美国历史最悠久的女子文理学院,在教育妇女从事科学研究方面有着悠久的历史。这一传统的一个关键组成部分是本科生和教师之间的研究项目的合作。为了更好地为学生未来的研究生涯做好准备,本科研究计划必须让学生尽可能多的直接,动手的技术和仪器的经验。这种仪器将通过使学生接触重要的现代生物化学技术来增强与PI合作的学生的研究经验。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Sean Decatur其他文献
Sean Decatur的其他文献
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{{ truncateString('Sean Decatur', 18)}}的其他基金
Collaborative Research: Development of 2D IR Spectroscopy as a Quantitative Probe of Protein Structure, with Applications to Membrane and Aggregated Proteins
合作研究:开发二维红外光谱作为蛋白质结构的定量探针,并应用于膜和聚集蛋白质
- 批准号:
0832591 - 财政年份:2008
- 资助金额:
$ 5.96万 - 项目类别:
Continuing Grant
RUI: Effects of Solvent-backbone Hydrogen Bonding and Backbone N-methylation on Peptide Conformation
RUI:溶剂-骨架氢键和骨架 N-甲基化对肽构象的影响
- 批准号:
0849164 - 财政年份:2008
- 资助金额:
$ 5.96万 - 项目类别:
Standard Grant
RUI: Effects of Solvent-backbone Hydrogen Bonding and Backbone N-methylation on Peptide Conformation
RUI:溶剂-骨架氢键和骨架 N-甲基化对肽构象的影响
- 批准号:
0719378 - 财政年份:2007
- 资助金额:
$ 5.96万 - 项目类别:
Standard Grant
Acquisition of Instrumentation for a Materials Characterization and Fabrication Facility
采购用于材料表征和制造设施的仪器
- 批准号:
0521052 - 财政年份:2005
- 资助金额:
$ 5.96万 - 项目类别:
Standard Grant
NUE: Integration of Nanotechnology into the Core Chemistry Curriculum at Mount Holyoke College
NUE:将纳米技术融入曼荷莲学院核心化学课程
- 批准号:
0407117 - 财政年份:2004
- 资助金额:
$ 5.96万 - 项目类别:
Standard Grant
Peptide aggregation, conformation, and dynamics via isotope-edited infrared spectroscopy
通过同位素编辑红外光谱研究肽聚集、构象和动力学
- 批准号:
0415878 - 财政年份:2004
- 资助金额:
$ 5.96万 - 项目类别:
Standard Grant
Acquisition of a Circular Dichroism Spectrometer
购买圆二色性光谱仪
- 批准号:
0079533 - 财政年份:2000
- 资助金额:
$ 5.96万 - 项目类别:
Standard Grant
CAREER: Infrared Spectroscopy of Synthetic and Semisynthetic Polypeptides
职业:合成和半合成多肽的红外光谱
- 批准号:
9984844 - 财政年份:2000
- 资助金额:
$ 5.96万 - 项目类别:
Continuing Grant
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