A Multi-User, Research and Training Ultracentrifugation Facility

这项提议是为了在约翰·霍普金斯大学霍姆伍德校区建立一个分析超速离心机设施。最初，这个设施将支持生物化学、生物物理学以及细胞和分子生物学领域的12名教职员工的研究。利用这一设备分析分子组件的流体力学性质，将与这些小组在校园内的生物热量计中心开展的工作高度互补。使用该设备的研究将包括：组蛋白和核小体；重复蛋白在信号转导中的功能；半乳糖抑制物；分子靶向和药物设计的新策略；RNA二十面体植物病毒的调节相互作用；植物木脂素对HIV-1和HSV-1感染的控制；以及葡萄球菌核酸酶的寡聚体。

This proposal is for the establishment of an analytical ultracentrifuge facility on the Homewood campus of Johns Hopkins University. Initially this facility will support the research of 12 faculty members in the areas of Biochemistry, Biophysics and Cell and Molecular Biology. The use of this facility for the analysis of the hydrodynamic properties of molecular assemblies will be highly complementary with the work carried out by these groups at the Biocalorimeter Center on campus. Research to use the facility will include studies of histones and nucleosomes; repeat-protein functions in signal transduction; the galactose repressor; new strategies for molecular targeting and drug design; regulatory interactions in RNA icosahedral plant viruses; control of HIV-1 and HSV-1 infection by plant lignans; and oligomers of staph nuclease.