Sequencing the Arabidopsis Genome

9872752该奖项为SPP（斯坦福-宾夕法尼亚大学-植物基因表达实验室/加州大学伯克利分校）联盟提供资金，继续对模式植物拟南芥的基因组进行测序。SPP成立的目的是对拟南芥的基因组进行测序，这项工作于1996年启动，是4个国家6个小组参与的多国项目的一部分。SPP联盟计划在未来三年内完成拟南芥1号染色体的序列。染色体的大小估计约为25mb（兆碱基）。测序的估计成本在每对碱基对0.40至0.50美元之间。持续监测测序过程的准确性，SPP已超过10,000个碱基中小于1个碱基不准确的可接受率。SPP联盟的即时数据发布政策规定，所有大于2kb的连续序列（contigs）都要发布到公共数据库，并提供有关完成程度的信息。这允许所有领域的研究科学家在尽可能早的时间访问序列数据，但有一些关于完成程度的标记。注释（相关的生物信息）在发布后尽快添加到GenBank条目中。每个SPP站点都建立并维护了自己的网页，其中包含当前进度以及每个站点的具体职责和进度的详细信息。此外，SPP联盟最近建立了一个新的网页，在一个地方总结了总体进展（http://www-sequence.stanford.edu/ara/SPP.html）。个人网站也可以从该地址访问。SPP的努力与其他测序小组的协调努力相结合，将有助于在2000年底前完成拟南芥基因组测序的目标。目前获得的部分拟南芥基因组序列信息已经为开花植物的生物学研究提供了新的见解。由于拟南芥是一种典型的开花植物，从拟南芥身上学到的东西很容易转移到所有的植物上。预计全基因组序列信息将给植物生物学领域带来革命性的变化。

9872752This award provides funds for the SPP (Stanford - University of Pennsylvania -Plant Gene Expression Laboratory/UC Berkeley) Consortium to continue sequencing the genome of the model plant, Arabidopsis thaliana. The SPP was established for the purpose of sequencing the genome of Arabidopsis thaliana and the effort was initiated in 1996 as part of the multinational project involving 6 groups in 4 nations. In this renewal award, the SPP Consortium plans to complete the sequence of chromosome 1 of Arabidopsis thaliana during the next three-year period. The size of the chromosome is estimated to be approximately 25 Mb (megabases). The estimated cost of sequencing is between $0.40 and $0.50 per base pair. The accuracy of the sequencing process is continually monitored and, the SPP has exceeded the accepted rate of less than 1 inaccurate base in 10,000 bases. The immediate data release policy of the SPP Consortium mandates that all contiguous sequences (contigs) greater than 2 kb be released to the public database with information on the degree of finishing. This allows research scientists in all fields to access the sequence data at the earliest possible time but with some notation as to the level of completion. Annotation (pertinent biological information) is added to the GenBank entries as soon as possible after release. Each SPP site has constructed and maintains its own web page with current progress and details about the specific duties and progress at each location. In addition, the SPP Consortium has recently built a new web page summarizing in one location the overall progress (http://www-sequence.stanford.edu/ara/SPP.html). The individual web sites can also be accessed from that address.The SPP efforts, combined with the coordinated efforts by the other sequencing groups, will contributed to the goal of completing the sequencing of the Arabidopsis genome by the end of the year 2000. Partial Arabidopsis genome sequence information obtained thus far has already contributed new insights into the biology of flowering plants. As Arabidopsis is a typical flowering plant, what is learned from Arabidopsis is readily transferable to all plants. It is expected that the complete genome sequence information will revolutionize the field of plant biology.