Evolution of Polydnavirus Genes in Microgastrine Braconid Parasitoid Wasps

小胃茧蜂寄生蜂多DNA病毒基因的进化

• 批准号: 9873748
• 负责人: James Whitfield
• 金额: $ 10万
• 依托单位: University of Arkansas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2002-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9873748&HistoricalAwards=false
• 关键词: Evolution; Polydnavirus; Genes; Microgastrine; Braconid

ABSTRACTPI: WhitfieldProposal: DEB 9873748University of Arkansas Of the parasitoid wasps important in attacking and regulating populations of caterpillars, perhaps the most familiar and numerically dominant are wasps belonging to the microgastroid complex. This complex of braconid wasps comprises eight subfamilies, several of which have already been demonstrated to carry hereditary polydnaviruses which compromise host Immune systems, and none of which have been shown not to carry the viruses. Since these viruses are crucial to the wasp/caterpillar parasitic relationship, they are of considerable evolutionary, physiological and economic interest. Phylogenetic analysis of braconid wasps based on comparative morphology and on 16S and 28S rRNA sequence data indicates that the microgastroids form a monophyletic lineage probably dating back at least 60 million years. Preliminary results of phylogenetic analysis of 16S and NADH1 DNA sequence data, from wasps in the genus Cotesia, and of the genes EP1 and Crv1 from the viruses they carry, suggest that the phylogeny of the polydnaviruses mirrors that of their wasp carriers, at least within this group. This project will more fully address whether this expected parallel phylogeny really exists by obtaining DNA sequences from the 16S, NADH1 and CO1 genes in the genomes of a further 25 species of microgastrines, and comparing the phylogeny obtained from these to that estimated from the EP1 and CrV1 genes of the viruses they carry. The results will have important implications for the use of phylogeny to predict aspects of the host-parasitoid relationship in this large, economically important group of wasps.

摘要PI：Whitfield 提案：DEB 9873748 阿肯色大学 在对攻击和调节毛毛虫种群具有重要作用的寄生蜂中，也许最熟悉且在数量上占主导地位的是属于小腹蜂复合体的黄蜂。 这种茧蜂复合体由八个亚科组成，其中几个亚科已被证明携带损害宿主免疫系统的遗传性多DNA病毒，并且没有一个亚科被证明不携带这种病毒。 由于这些病毒对黄蜂/毛毛虫寄生关系至关重要，因此它们具有相当大的进化、生理和经济意义。基于比较形态学和 16S 和 28S rRNA 序列数据的对茧蜂的系统发育分析表明，小腹蜂形成的单系谱系可能可以追溯到至少 6000 万年前。 对 Cotesia 属黄蜂的 16S 和 NADH1 DNA 序列数据以及它们携带的病毒的 EP1 和 Crv1 基因进行系统发育分析的初步结果表明，多 DNA 病毒的系统发育反映了其黄蜂携带者的系统发育，至少在该组中是这样。该项目将通过从另外 25 个小胃动物的基因组中的 16S、NADH1 和 CO1 基因获取 DNA 序列，并将从这些序列中获得的系统发育与从它们携带的病毒的 EP1 和 CrV1 基因估计的系统发育进行比较，更全面地解决这种预期的平行系统发育是否真的存在。 这些结果将对利用系统发育来预测这一具有重要经济意义的大型黄蜂群体中宿主与寄生蜂的关系具有重要意义。