Host integration by a symbiotic polydnavirus

共生多DNA病毒的宿主整合

• 批准号: 1145953
• 负责人: Michael Strand
• 金额: $ 39.9万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1145953&HistoricalAwards=false
• 关键词: Host; integration; symbiotic; polydnavirus

Understanding how animals and plants interact with microorganisms is critically important to basic biology, agriculture, and medicine. Obligate beneficial symbioses refer to interactions where a microorganism can only live in a particular animal or plant, and reciprocally the animal or plant can only survive if the microorganism is present. Most known beneficial symbionts of insects are bacteria. However, another large and important symbiotic association occurs between a family of viruses named the Polydnaviridae and parasitoid wasps, which parasitize and consume other types of insects. This biology makes parasitoid wasps very important control agents of insects, including many species that are economically harmful to humans. Polydnavirus-carrying wasps can survive in wasps only if they introduce the virus into parasitized hosts. Recent results from the PI's laboratory indicate that polydnaviruses function in parasitized hosts by rapidly integrating their DNA genomes into the genome of the host. However, the mechanism by which this integration event occurs is unknown. The goal of this project is to understand how polydnavirus-mediated integration occurs. This project will characterize the location in the host genome where the viral genome inserts itself. Functional experiments will also be conducted to identify the molecules that control the integration process. In addition to enhancing understanding of polydnaviruses, wasps and hosts, the proposed research will provide insights into a new mechanism of moving genes from one organism to another. The work will promote scientific training and education by providing independent research opportunities for postdoctoral, graduate and undergraduate students. Results from the study will also be used to enhance classroom instruction at the high school and university level.

了解动物和植物如何与微生物相互作用对基础生物学，农业和医学至关重要。专性有益共生是指微生物只能在特定的动物或植物中生存的相互作用，并且只有当微生物存在时，动物或植物才能生存。大多数已知的有益昆虫共生体是细菌。然而，另一个大的和重要的共生关系发生在一个名为多DNA病毒科的病毒家族和寄生蜂之间，寄生蜂寄生并消耗其他类型的昆虫。这种生物学特性使得寄生蜂成为非常重要的昆虫控制媒介，包括许多对人类经济有害的物种。携带多脱氧核糖核酸病毒的黄蜂只有在将病毒引入寄生宿主时才能在黄蜂体内存活。PI实验室的最新结果表明，多DNA病毒通过将其DNA基因组快速整合到宿主基因组中来在寄生宿主中发挥作用。然而，这种整合事件发生的机制尚不清楚。该项目的目标是了解多DNA病毒介导的整合是如何发生的。该项目将描述病毒基因组插入宿主基因组的位置。还将进行功能实验以鉴定控制整合过程的分子。除了增强对多DNA病毒、黄蜂和宿主的了解外，这项拟议中的研究还将深入了解将基因从一种生物体转移到另一种生物体的新机制。这项工作将通过为博士后、研究生和本科生提供独立研究机会，促进科学培训和教育。研究结果还将用于加强高中和大学的课堂教学。