CAREER: Rational Manipulation of Fungal Cell Wall Composition to Control Hyphal Fragmentation

职业：合理调控真菌细胞壁组成以控制菌丝断裂

• 批准号: 9876012
• 负责人: Mark Marten
• 金额: $ 20万
• 依托单位: University of Maryland Baltimore County
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9876012&HistoricalAwards=false
• 关键词: CAREER; Rational; Manipulation; Fungal; Cell

The goal of this proposal is to obtain a better understanding of how fungal cell wall composition and structure affect cellular physical properties. The results could lead to new and/or improved antibiotic fermentations and a better understanding of fungus/plant interactions. A model system using Aspergillus nidulans will be studied observing cell wall compositions (chitin, B-glucan, protein content, protein expression pattern) and physical properties (tensile strength, wall thickness, hyphal diameter) and correlating these properties with composition. A fungal strain with minimal hyphal fragmentation will be genetically engineered using the above relationships. The educational plan centers on the use of an engineer/life-scientist/industry collaboration to promote active, hands-on learning in the bioengineering field.

该提案的目标是更好地了解真菌细胞壁的组成和结构如何影响细胞的物理性质。这些结果可能导致新的和/或改进的抗生素发酵，并更好地了解真菌/植物相互作用。将研究使用构巢曲霉的模型系统，观察细胞壁组成（几丁质、B-葡聚糖、蛋白质含量、蛋白质表达模式）和物理性质（拉伸强度、壁厚度、菌丝直径），并将这些性质与组成相关联。具有最小菌丝片段化的真菌菌株将使用上述关系进行遗传工程改造。教育计划的中心是利用工程师/生命科学家/行业合作，促进生物工程领域的积极，动手学习。