Toxicology, Insect Specificity, and Chemistry of a Novel Peptidic Insecticide - TMOF

该项目由“研究中心-小企业协同研发”计划支持。这项研究是由美国国家科学基金会（NSF）和世界自然与自然保护委员会（IUCRC）的害虫综合管理中心和北卡罗来纳州达勒姆的一家小公司昆虫生物技术公司联合进行的。昆虫生物技术公司（IBI）正在开发一种新的生物杀虫剂技术（胰蛋白酶调节Oostatic Factor， TMOF），该技术具有靶向特异性，对非目标生物无毒，并且在环境中具有半衰期短的可生物降解性。在本研究中，将发展技术以确定在特定昆虫物种中TMOF机制的存在，对TMOF化合物进行生物测定，并分离和表征活性TMOF化学物种。为了模拟这些新的TMOFs而设计的化合物将被合成并测试其活性。IBI将派遣一名博士后实习生到中心工作。中心和IBI的工作人员将每两个月举行一次会议。IBI将制备和检测TMOF肽类似物。这项技术代表了一种新的害虫控制工具，具有广泛的适用性，与环境和健康安全相结合，是环境保护局颁布的新的《食品质量保护法》规定下管理选择的关键。该项目为IBI提供了与中心主要企业成员互动的机会（反之亦然），以加强IBI技术与新管理方案和旧主流产品的结合。该公司基于肽的技术有可能成为下一代生物安全杀虫剂之一。

EEC-9903837Stinner/North Carolina State U.This project is supported under the initiative on "Research Centers - Small Firms Collaborative R&D." The study concerns joint research between the Center for Integrated Pest Management, an NSF IUCRC, and Insect Biotechnology, Inc., a small firm in Durham, NC. Insect Biotechnology, Inc. (IBI) is developing a new, biorational insecticide technology (Trypsin Modulating Oostatic Factor, TMOF) that is target specific, nontoxic to nontarget organisms, and biodegradable with a short half-life in the environment. In the present study, techniques will be developed to ascertain the presence of the TMOF mechanism in a given insect species, to perform bioassays for the TMOF compound(s) and isolate and characterize the active TMOF chemical species. Chemical compounds designed to mimic these new TMOFs will be synthesized and tested for activity. IBI will assign one postdoctoral trainee to work in the Center. Personnel of the Center and IBI will hold bimonthly meetings. IBI will prepare and assay TMOF peptide analogues. This technology represents a new pest control tool with wide applicability, coupled with environmental and health safety and is a key to management options under the new Food Quality Protection Act regulations being promulgated by EPA. This project offers IBI the opportunity to interact with key corporate members of the Center (and vice versa) to enhance the incorporation of IBI's technology into new management schemes and older mainstream products. The firm's peptide-based technology has a potential to be one of the next generation of biorational, safe insecticides.